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Molecular Genetic Studies on Myxobolus cylindricus and Henneguya mystasi (Myxosporea: Myxobolidae) Infecting Two Indian Fish Species, Channa gachua and Mystus vittatus, Respectively

Chaudhary, Anshu and Gupta, A. and Goswami, U. and Cech, Gábor and Molnár, Kálmán and Singh, H. S. and Székely, Csaba (2019) Molecular Genetic Studies on Myxobolus cylindricus and Henneguya mystasi (Myxosporea: Myxobolidae) Infecting Two Indian Fish Species, Channa gachua and Mystus vittatus, Respectively. ACTA PARASITOLOGICA, 64 (1). pp. 129-137. ISSN 1230-2821

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Abstract

Introduction Myxozoan infections of Indian freshwater fshes are relatively well studied, but their validity is supported with molecular and phylogenetic data only for a few species. Objectives The primary objective was to present molecular data for two Myxozoan species, Myxobolus cylindricus and Henneguya gachua collected from Indian freshwater fshes, the dwarf snakehead Channa gachua and the striped dwarf catfsh Mystus vittatus, respectively. Methods Various organs of 56 C. gachua and 48 M. vittatus were dissected. Myxozoan plasmodia with mature spores were collected from the gills under a dissecting microscope. Spores obtained from plasmodia from infected hosts were fxed in 80% ethanol in vials and sent for further morphological and molecular examinations to Hungary. The 18S rDNA gene of Myxobolus and Henneguya spp. was amplifed using nested polymerase chain reaction (PCR). Phylogenetic analysis was performed using maximum likelihood (ML) and Bayesian inference (BI) methods. Results Morphological characteristics of M. cylindricus and H. mystasi spores corresponded to the original descriptions made by Sarkar, Mazumdar and Pramanik, 1985 and Haldar, Samal, and Mukhopadhyay, 1997, respectively. Phylogenetic analysis of the 18S rDNA gene revealed that the sequences of M. cylindricus difered from those of most Indian Myxobolus sp., known mostly from cyprinid fshes and formed a subgroup with Myxobolus neurophilus, a parasite of a perciform host, and with Henneguya chaudhuryi, a species belonging to a diferent genus but described from a closely related channid host. It was also closely related to another Henneguya species, H. lesteri, described from Sillago analis, a coastal fsh. Henneguya mystasi had the closest similarity to Henneguya bicaudi, a species described from an Indian cyprinid fsh and to Henneguya pellucida reported from a characid fsh known from South America. Conclusion Molecular data received by us gives a solid basis for further identifcation of these myxozoans, the pathogenicity of which probably plays an economic role at culturing the hosts.

Item Type: Article
Subjects: Q Science / természettudomány > QL Zoology / állattan
Q Science / természettudomány > QL Zoology / állattan > QL01 Systematic zoology / állatrendszertan
S Agriculture / mezőgazdaság > SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás, halászat, sporthorgászat
Depositing User: Dr. Csaba Székely
Date Deposited: 24 Sep 2019 14:51
Last Modified: 24 Sep 2019 14:51
URI: http://real.mtak.hu/id/eprint/100150

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