Majoros, Hajnalka and Újfaludi, Zsuzsanna and Borsos, Barbara Nikolett and Hudacsek, Viktória Vivien and Nagy, Zita and Buzás, Krisztina and Boros, Imre Miklós and Pankotai, Tibor (2019) SerpinB2 is involved in cellular response upon UV irradiation. SCIENTIFIC REPORTS, 9 (1). ISSN 2045-2322
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Abstract
Ultraviolet light induced pyrimidine dimer is a helix distortion DNA damage type, which recruits repair complexes. However, proteins of these complexes that take part in both DNA damage recognition and repair have been well-described, the regulation of the downstream steps of nucleotide excision repair (NER) have not been clearly clarified yet. In a high-throughput screen, we identified SerpinB2 (SPB2) as one of the most dramatically upregulated gene in keratinocytes following UV irradiation. We found that both the mRNA and the protein levels of SPB2 were increased upon UV irradiation in various cell lines. Additionally, UV damage induced translocation of SPB2 from the cytoplasm to the nucleus as well as the damage induced foci formation of it. Here we show that SPB2 co-localizes with XPB involved in the NER pathway at UV-induced repair foci. Finally, we demonstrated that UV irradiation promoted the association of SPB2 with ubiquitylated proteins. In basal cell carcinoma tumour cells, we identified changes in the subcellular localization of SPB2. Based on our results, we conclude that SPB2 protein has a novel role in UV-induced NER pathway, since it regulates the removal of the repair complex from the damaged site leading to cancerous malformation.
| Item Type: | Article |
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| Additional Information: | Funding Agency and Grant Number: Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences; National Research, Development and Innovation Office [GINOP-2.3.2-15-2016-00020, GINOP-2.3.2-15-2016-00036] Funding text: This work was sponsored by the Janos Bolyai Research Scholarship of the Hungarian Academy of Sciences (T.P.) and the National Research, Development and Innovation Office Grants GINOP-2.3.2-15-2016-00020 and GINOP-2.3.2-15-2016-00036. Department of Biochemistry and Molecular Biology, University of Szeged, Faculty of Science and Informatics, Szeged, Hungary Institute of Biochemistry, Biological Research Centre, Szeged, Hungary Department of Functional Genomics and Cancer, Institute of Genetics and Molecular and Cellular Biology (IGBMC), Illkirch, France Department of Pathology of the Gyula Kenézy University Hospital, University of Debrecen, Debrecen, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Hungarian Center of Excellence for Molecular Medicine, Szeged, Hungary Export Date: 13 May 2019 Correspondence Address: Pankotai, T.; Department of Biochemistry and Molecular Biology, University of Szeged, Faculty of Science and InformaticsHungary; email: pankotai@bio.u-szeged.hu Department of Biochemistry and Molecular Biology, University of Szeged, Faculty of Science and Informatics, Szeged, Hungary Institute of Biochemistry, Biological Research Centre, Szeged, Hungary Department of Functional Genomics and Cancer, Institute of Genetics and Molecular and Cellular Biology (IGBMC), Illkirch, France Department of Pathology of the Gyula Kenézy University Hospital, University of Debrecen, Debrecen, Hungary Department of Immunology, Faculty of Medicine, University of Debrecen, Debrecen, Hungary Hungarian Center of Excellence for Molecular Medicine, Szeged, Hungary Export Date: 30 August 2019 Correspondence Address: Pankotai, T.; Department of Biochemistry and Molecular Biology, University of Szeged, Faculty of Science and InformaticsHungary; email: pankotai@bio.u-szeged.hu Funding details: Magyar Tudományos Akadémia, MTA Funding details: Office of Research, Innovation and Economic Development, California State Polytechnic University, Pomona, GINOP-2.3.2-15-2016-00036, GINOP-2.3.2-15-2016-00020 Funding text 1: This work was sponsored by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences (T.P.) and the National Research, Development and Innovation Office Grants GINOP-2.3.2-15-2016-00020 and GINOP-2.3.2-15-2016-00036. |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 24 Sep 2019 17:02 |
| Last Modified: | 24 Sep 2019 17:02 |
| URI: | http://real.mtak.hu/id/eprint/101050 |
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