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Interaction Force Fluctuations in Antigen-Antibody Biorecognition Studied by Atomic Force Spectroscopy

Bizzarri, Anna Rita and Végh, Attila Gergely and Váró, György and Cannistraro, Salvatore (2019) Interaction Force Fluctuations in Antigen-Antibody Biorecognition Studied by Atomic Force Spectroscopy. ACS OMEGA, 4 (2). pp. 3627-3634. ISSN 2470-1343

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Abstract

The formation of a specific complex between beta(2)mu globulin and anti beta(2)mu globulin was investigated by analyzing the force fluctuations recorded in an atomic force spectroscopy biorecognition experiment. We found that a 1/f noise appears in the power spectra of force fluctuations when the tip, functionalized with beta(2)mu globulin, reaches a distance of 0.50 nm from the partner-charged substrate while a specific biorecognition process occurs. Concomitantly, in this active region, the distribution of the times spent by the tip in the proximity of the substrate exhibits a power law trend characterized by a longtime tail. All of these findings are put into relationship to a slowing down of the energy landscape exploration, consistent with a restricted sampling dynamics of the conformational states driving to the final binding state. The hypothesis that a combination of a conformational substrate and an induced fit hybrid binding mechanism controls the specific complex formation is put forward and discussed also in connection with the fluctuations of the hydration water network.

Item Type: Article
Uncontrolled Keywords: LOCALIZATION; KINETICS; DYNAMICS; NOISE; MICROSCOPE; POTENTIAL-ENERGY; Conformational selection; protein-protein; RECOGNITION EVENTS;
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3020 Biophysics / biofizika
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 21 Nov 2019 08:04
Last Modified: 20 Apr 2023 10:38
URI: http://real.mtak.hu/id/eprint/103439

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