REAL

Membrane protein megahertz crystallography at the European XFEL

Gisriel, Chris and Coe, Jesse and Letrun, Romain and Yefanov, Oleksandr M. and Luna-Chavez, Cesar and Brockhauser, Sándor (2019) Membrane protein megahertz crystallography at the European XFEL. NATURE COMMUNICATIONS, 10. ISSN 2041-1723

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Abstract

The world's first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulses. With its rapid pulse rate, the European XFEL may alleviate some of the increasing demand for XFEL beamtime, particularly for membrane protein serial femtosecond crystallography (SFX), leveraging orders-of-magnitude faster data collection. Here, we report the first membrane protein megahertz SFX experiment, where we determined a 2.9 angstrom-resolution SFX structure of the large membrane protein complex, Photosystem I, a > 1 MDa complex containing 36 protein subunits and 381 cofactors. We address challenges to megahertz SFX for membrane protein complexes, including growth of large quantities of crystals and the large molecular and unit cell size that influence data collection and analysis. The results imply that megahertz crystallography could have an important impact on structure determination of large protein complexes with XFELs.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3020 Biophysics / biofizika
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 22 Nov 2019 14:59
Last Modified: 22 Nov 2019 14:59
URI: http://real.mtak.hu/id/eprint/103600

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