Mycoplasma anserisalpingitis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology

Volokhov, Dmitriy V. and Grózner, Dénes and Gyuranecz, Miklós and Ferguson-Noel, Naola and Gao, Yamei and Stipkovits, László (2020) Mycoplasma anserisalpingitis sp. nov., isolated from European domestic geese (Anser anser domesticus) with reproductive pathology. International Journal of Systematic and Evolutionary Microbiology. pp. 1-29. ISSN 1466-5026 (print); 1466-5034 (online) (Submitted)

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Abstract

In 1983 Mycoplasma sp. strain 1220 was isolated in Hungary from phallus lymph specimen of a gander with phallus inflammation. During the period from 1983 through 2017, Mycoplasma sp. 1220 was also identified and isolated from the trachea, airsac, lung, liver, ovary, testis, peritoneum, cloaca of diseased geese in several countries. Sixteen studied strains produce acid from glucose and fructose but did not hydrolyzed arginine or urea, and all were propagated under aerobic, microaerophilic and anaerobic atmospheric conditions at +35-37˚C using either SP4 or PPLO medium supplemented with glucose. Colonies on solid medium show a typical fried-egg appearance and transmission electron microscopy revealed a typical mycoplasma cellular morphology. Molecular characterization included assessment of the following genetic loci: 16S rRNA, 23S rRNA, 16S‐23S rRNA ITS, rpoB, rpoC, rpoD, uvrA, parC, topA, dnaE, fusA, and pyk. The genome was sequenced for type strain 1220T. The 16S rRNA gene sequences of studied strains of Mycoplasma sp. 1220 shared 99.02-99.19% nucleotide similarity with M. anatis strains but demonstrated ≤95.00-96.70% nucleotide similarity to the 16S rRNA genes of other species of the genus Mycoplasma. Phylogenetic, average nucleotide and amino acid identities (ANI/AAI) analyses revealed that the novel species was most closely related to M. anatis. Based on these data we propose a novel species of the genus Mycoplasma, for which the name Mycoplasma anserisalpingitis sp. nov. is proposed with the type strain 1220T (=ATCC BAA-2147T =NCTC 13513T =DSM 23982T). The G+C content is 26.70%, genome size is 959,110 bp.

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