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Investigation of sphingosin-1-phosphate-triggered matriptase activation using a rat primary hepatocyte model

Barna, Réka Fanni and Pomothy, Judit Mercédesz and Paréj, Zsuzsanna and Pásztiné Gere, Erzsébet (2019) Investigation of sphingosin-1-phosphate-triggered matriptase activation using a rat primary hepatocyte model. Acta Veterinaria Hungarica, 67 (4). pp. 578-587. ISSN 0236-6290

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Abstract

Sphingosine-1-phosphate (S1P) has been reported as a matriptase activator. The aim of this study was to reveal if S1P can influence hepcidin production. Furthermore, we investigated how S1P can affect the viability and the redox status of primary hepatocytes. Rat primary hepatocytes were cultivated for 72 h and were treated with 50, 200, 1000 ng/ml S1P. Cell-free supernatants were collected every 24 h. Cell viability was tested by a colorimetric method using tetrazolium compound (MTS). The hepcidin levels in the cell-free supernatants were examined with hepcidin sandwich ELISA to determine the effect of S1P on the hepcidin-modulating ability of matriptase. In order to estimate the extent of S1P-generated oxidative stress, extracellular H<sub>2</sub>O<sub>2</sub> measurements were performed by the use of fluorescent dye. Based on the findings, S1P treatment did not cause cell death for 72 h at concentrations up to 1000 ng/ml. S1P did not influence the extracellular H<sub>2</sub>O<sub>2</sub> production for 72 h. The hepcidin levels were significantly suppressed in hepatocytes exposed to S1P treatment. Further studies would be needed to explore the exact mechanism of action of S1P.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: László Sallai-Tóth
Date Deposited: 18 Feb 2020 08:54
Last Modified: 31 Dec 2020 00:42
URI: http://real.mtak.hu/id/eprint/106350

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