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Digenean Holostephanus (Trematoda: Digenea:Cyathocotylidae) metacercariae in commoncarp (Cyprinus carpioLinnaeus, 1758) muscle: zoonotic potential and sensitivity tophysico-chemical treatments

Sándor, D. and Gyöngy, M. and Nyeste, K. and Czeglédi, I. and Székely, Cs. and Cech, G. (2020) Digenean Holostephanus (Trematoda: Digenea:Cyathocotylidae) metacercariae in commoncarp (Cyprinus carpioLinnaeus, 1758) muscle: zoonotic potential and sensitivity tophysico-chemical treatments. Journal of Helminthology, 94. pp. 1-8. ISSN 0022-149X, 1475-2697

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Abstract

Metacercariae of various species within the genus Holostephanus Szidat, 1936 (Trematoda: Digenea: Cyathocotylidae) occur in muscles of both farmed and wild fish, including common carp (Cyprinus carpio Linnaeus, 1758). The life cycle includes a snail as first intermediate host, fish as second intermediate host and birds or mammals as final hosts. We studied the zoonotic potential and the viability of Holostephanus metacercariae from common carp following exposure to various physical and chemical treatments. Muscle tissue samples of common carp specimens from a fish farm in the north-eastern part of Hungary were examined and metacercariae recovered. The zoonotic potential was evaluated experimentally by using small mammals as models (albino mice, n = 2; and Syrian hamsters, n = 4) infected per os with Holostephanus cysts. Parallelly, Metagonimus metacercariae were used as positive controls. We could not confirm the zoonotic potential of Holostephanus metacercariae as they did not survive in the mammalian intestine whereas Metagonimus metacercariae developed to the adult stage. Weassessed the viability of metacercariae isolated from common carp specimens during exposure to different physical treatments (temperatures of −18°C, +20°C, +40°C and +60°C) and chemical agents (5% and 10% acetic acid and 10% sodium chloride (NaCl)). Metacercariae lost viability by freezing at −18°C (2 h), heating at 60°C (20 min), incubation in 5% and 10% acetic acid (5 min) and 10% NaCl (2 h). These methods served as models to investigate the effectiveness of food preparation techniques (such as cold and hot smoking, freezing, salting and pickling) on the survival of metacercariae.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > SH Aquaculture. Fisheries. Angling / vizi növény-és állattartás, halászat, sporthorgászat
S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: Dr Gábor Cech
Date Deposited: 07 Apr 2020 10:54
Last Modified: 17 Jul 2020 23:15
URI: http://real.mtak.hu/id/eprint/107865

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