Sugár, Simon and Turiák, Lilla and Vékey, Károly and Drahos, László (2019) Widespread presence of bovine proteins in human cell lines. JOURNAL OF MASS SPECTROMETRY. ISSN 1076-5174
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Official URL: https://doi.org/10.1002/jms.4464
Abstract
HPLC-MS/MS analysis of various human cell lines show the presence of a major amount of bovine protein contaminants. These likely originate from fetal bovine serum (FBS), typically used in cell cultures. If evaluated against a human protein database, on average 10% of the identified human proteins will be misleading (bovine proteins, but indicated as if they were human). Bovine contaminants therefore may cause major bias in proteomic studies of cell cultures, if not considered explicitly.
| Item Type: | Article |
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| Subjects: | Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia |
| Depositing User: | Dr. Lilla Turiák |
| Date Deposited: | 23 Apr 2020 11:26 |
| Last Modified: | 07 Nov 2020 00:15 |
| URI: | http://real.mtak.hu/id/eprint/108223 |
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