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Rapid and quantitative antimalarial drug efficacy testing via the magneto-optical detection of hemozoin

Molnár, Petra and Orbán, Ágnes and Izrael, Richard and Babai, Réka and Marton, Lívia and Butykai, Ádám and G. Vértessy, Beáta and Kézsmárki, István (2020) Rapid and quantitative antimalarial drug efficacy testing via the magneto-optical detection of hemozoin. SCIENTIFIC REPORTS, 10. pp. 1-11. ISSN 2045-2322

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Abstract

Emergence of resistant Plasmodium species makes drug efficacy testing a crucial part of malaria control. Here we describe a novel assay for sensitive, fast and simple drug screening via the magneto-optical detection of hemozoin, a natural biomarker formed during the hemoglobin metabolism of Plasmodium species. By quantifying hemozoin production over the intraerythrocytic cycle, we reveal that hemozoin formation is already initiated by ~ 6–12 h old ring-stage parasites. We demonstrate that the new assay is capable of drug efficacy testing with incubation times as short as 6–10 h, using synchronized P. falciparum 3D7 cultures incubated with chloroquine, piperaquine and dihydroartemisinin. The determined 50% inhibitory concentrations agree well with values established by standard assays requiring significantly longer testing time. Accordingly, we conclude that magneto-optical hemozoin detection provides a practical approach for the quick assessment of drug effect with short incubation times, which may also facilitate stage-specific assessment of drug inhibitory effects. © 2020, The Author(s).

Item Type: Article
Subjects: R Medicine / orvostudomány > RS Pharmacy and materia medica / gyógyszerészet, gyógyászati eszközök
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 22 Sep 2020 14:13
Last Modified: 22 Sep 2020 14:13
URI: http://real.mtak.hu/id/eprint/114053

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