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Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke

Szalay, Gergely and Martinecz, Bernadett and Lénárt, Nikolett and Környei, Zsuzsanna and Orsolits, Barbara and Judák, Linda and Császár, Eszter and Fekete, Rebeka and Katona, Gergely and Rózsa J., Balázs and Dénes, Ádám (2016) Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke. NATURE COMMUNICATIONS, 7. No.-11499. ISSN 2041-1723

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Abstract

Microglia are the main immune cells of the brain and contribute to common brain diseases. However, it is unclear how microglia influence neuronal activity and survival in the injured brain in vivo. Here we develop a precisely controlled model of brain injury induced by cerebral ischaemia combined with fast in vivo two-photon calcium imaging and selective microglial manipulation. We show that selective elimination of microglia leads to a striking, 60% increase in infarct size, which is reversed by microglial repopulation. Microglia-mediated protection includes reduction of excitotoxic injury, since an absence of microglia leads to dysregulated neuronal calcium responses, calcium overload and increased neuronal death. Furthermore, the incidence of spreading depolarization (SD) is markedly reduced in the absence of microglia. Thus, microglia are involved in changes in neuronal network activity and SD after brain injury in vivo that could have important implications for common brain diseases.

Item Type: Article
Subjects: R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry / idegkórtan, neurológia, pszichiátria
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 24 Sep 2020 07:24
Last Modified: 24 Sep 2020 07:24
URI: http://real.mtak.hu/id/eprint/114296

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