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Cellular architecture and transmitter phenotypes of neurons of the mouse median raphe region

Sós, Katalin Eszter and Mayer, Márton István and Cserép, Csaba and Takacs, F. S. and Szőnyi, András and Freund, Tamás and Nyíri, Gábor (2017) Cellular architecture and transmitter phenotypes of neurons of the mouse median raphe region. BRAIN STRUCTURE & FUNCTION, 222 (1). pp. 287-299. ISSN 1863-2653

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Abstract

The median raphe region (MRR, which consist of MR and paramedian raphe regions) plays a crucial role in regulating cortical as well as subcortical network activity and behavior, while its malfunctioning may lead to disorders, such as schizophrenia, major depression, or anxiety. Mouse MRR neurons are classically identified on the basis of their serotonin (5-HT), vesicular glutamate transporter type 3 (VGLUT3), and gamma-aminobutyric acid (GABA) contents; however, the exact cellular composition of MRR regarding transmitter phenotypes is still unknown. Using an unbiased stereological method, we found that in the MR, 8.5 % of the neurons were 5-HT, 26 % were VGLUT3, and 12.8 % were 5-HT and VGLUT3 positive; whereas 37.2 % of the neurons were GABAergic, and 14.4 % were triple negative. In the whole MRR, 2.1 % of the neurons were 5-HT, 7 % were VGLUT3, and 3.6 % were 5-HT and VGLUT3 positive; whereas 61 % of the neurons were GABAergic. Surprisingly, 25.4 % of the neurons were triple negative and were only positive for the neuronal marker NeuN. PET-1/ePET-Cre transgenic mouse lines are widely used to specifically manipulate only 5-HT containing neurons. Interestingly, however, using the ePET-Cre transgenic mice, we found that far more VGLUT3 positive cells expressed ePET than 5-HT positive cells, and about 38 % of the ePET cells contained only VGLUT3, while more than 30 % of 5-HT cells were ePET negative. These data should facilitate the reinterpretation of PET-1/ePET related data in the literature and the identification of the functional role of a putatively new type of triple-negative neuron in the MRR.

Item Type: Article
Additional Information: Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :11 Export Date: 23 July 2019 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of SciencesHungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: serotonin, 50-67-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; Amino Acid Transport Systems, Acidic; gamma-Aminobutyric Acid; Serotonin; Transcription Factors; vesicular glutamate transporter 3, mouse Funding details: European Research Council, ERC-2011-ADG-294313 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001, MOP 4.2.4 Funding details: National Institutes of Health, NS030549 Funding text 1: We thank Em?ke Sz??pn?? Simon, Katalin Lengyel and Katalin Iv??nyi for the excellent technical assistance. The authors wish to thank L??szl?? Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the European Research Council (Grant number ERC-2011-ADG-294313, SERRACO) and the National Institutes of Health (Grant number NS030549). G??bor Nyiri was supported by a J??nos Bolyai Research Scholarship. Andr??s Sz?nyi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of T??MOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program". Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :11 Export Date: 3 August 2019 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of SciencesHungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: serotonin, 50-67-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; Amino Acid Transport Systems, Acidic; gamma-Aminobutyric Acid; Serotonin; Transcription Factors; vesicular glutamate transporter 3, mouse Funding details: European Research Council, ERC-2011-ADG-294313 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001, MOP 4.2.4 Funding details: National Institutes of Health, NS030549 Funding text 1: We thank Em?ke Sz??pn?? Simon, Katalin Lengyel and Katalin Iv??nyi for the excellent technical assistance. The authors wish to thank L??szl?? Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the European Research Council (Grant number ERC-2011-ADG-294313, SERRACO) and the National Institutes of Health (Grant number NS030549). G??bor Nyiri was supported by a J??nos Bolyai Research Scholarship. Andr??s Sz?nyi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of T??MOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program". Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :11 Export Date: 16 August 2019 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of SciencesHungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: serotonin, 50-67-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; Amino Acid Transport Systems, Acidic; gamma-Aminobutyric Acid; Serotonin; Transcription Factors; vesicular glutamate transporter 3, mouse Funding details: European Research Council, ERC-2011-ADG-294313 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001, MOP 4.2.4 Funding details: National Institutes of Health, NS030549 Funding text 1: We thank Em?ke Sz??pn?? Simon, Katalin Lengyel and Katalin Iv??nyi for the excellent technical assistance. The authors wish to thank L??szl?? Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the European Research Council (Grant number ERC-2011-ADG-294313, SERRACO) and the National Institutes of Health (Grant number NS030549). G??bor Nyiri was supported by a J??nos Bolyai Research Scholarship. Andr??s Sz?nyi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of T??MOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program". Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :11 Export Date: 28 August 2019 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of SciencesHungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: serotonin, 50-67-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; Amino Acid Transport Systems, Acidic; gamma-Aminobutyric Acid; Serotonin; Transcription Factors; vesicular glutamate transporter 3, mouse Funding details: European Research Council, ERC-2011-ADG-294313 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001, MOP 4.2.4 Funding details: National Institutes of Health, NS030549 Funding text 1: We thank Em?ke Sz??pn?? Simon, Katalin Lengyel and Katalin Iv??nyi for the excellent technical assistance. The authors wish to thank L??szl?? Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the European Research Council (Grant number ERC-2011-ADG-294313, SERRACO) and the National Institutes of Health (Grant number NS030549). G??bor Nyiri was supported by a J??nos Bolyai Research Scholarship. Andr??s Sz?nyi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of T??MOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program". Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :19 Export Date: 13 May 2020 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of SciencesHungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: serotonin, 50-67-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; Amino Acid Transport Systems, Acidic; gamma-Aminobutyric Acid; Serotonin; Transcription Factors; vesicular glutamate transporter 3, mouse Funding details: European Research Council, ERC, ERC-2011-ADG-294313 Funding details: National Institutes of Health, NIH, NS030549 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001 Funding details: European Commission, EC Funding text 1: We thank Em?ke Sz??pn?? Simon, Katalin Lengyel and Katalin Iv??nyi for the excellent technical assistance. The authors wish to thank L??szl?? Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the European Research Council (Grant number ERC-2011-ADG-294313, SERRACO) and the National Institutes of Health (Grant number NS030549). G??bor Nyiri was supported by a J??nos Bolyai Research Scholarship. Andr??s Sz?nyi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of T??MOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program". Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :19 Export Date: 23 May 2020 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of SciencesHungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: serotonin, 50-67-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; Amino Acid Transport Systems, Acidic; gamma-Aminobutyric Acid; Serotonin; Transcription Factors; vesicular glutamate transporter 3, mouse Funding details: European Research Council, ERC, ERC-2011-ADG-294313 Funding details: National Institutes of Health, NIH, NS030549 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001 Funding details: European Commission, EC Funding text 1: We thank Em?ke Sz??pn?? Simon, Katalin Lengyel and Katalin Iv??nyi for the excellent technical assistance. The authors wish to thank L??szl?? Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the European Research Council (Grant number ERC-2011-ADG-294313, SERRACO) and the National Institutes of Health (Grant number NS030549). G??bor Nyiri was supported by a J??nos Bolyai Research Scholarship. Andr??s Sz?nyi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of T??MOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program". Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :19 Export Date: 24 May 2020 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of SciencesHungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: serotonin, 50-67-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; Amino Acid Transport Systems, Acidic; gamma-Aminobutyric Acid; Serotonin; Transcription Factors; vesicular glutamate transporter 3, mouse Funding details: European Research Council, ERC, ERC-2011-ADG-294313 Funding details: National Institutes of Health, NIH, NS030549 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001 Funding details: European Commission, EC Funding text 1: We thank Em?ke Sz??pn?? Simon, Katalin Lengyel and Katalin Iv??nyi for the excellent technical assistance. The authors wish to thank L??szl?? Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the European Research Council (Grant number ERC-2011-ADG-294313, SERRACO) and the National Institutes of Health (Grant number NS030549). G??bor Nyiri was supported by a J??nos Bolyai Research Scholarship. Andr??s Sz?nyi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of T??MOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program". Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :19 Export Date: 25 May 2020 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of SciencesHungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: serotonin, 50-67-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; Amino Acid Transport Systems, Acidic; gamma-Aminobutyric Acid; Serotonin; Transcription Factors; vesicular glutamate transporter 3, mouse Funding details: European Research Council, ERC, ERC-2011-ADG-294313 Funding details: National Institutes of Health, NIH, NS030549 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001 Funding details: European Commission, EC Funding text 1: We thank Em?ke Sz??pn?? Simon, Katalin Lengyel and Katalin Iv??nyi for the excellent technical assistance. The authors wish to thank L??szl?? Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the European Research Council (Grant number ERC-2011-ADG-294313, SERRACO) and the National Institutes of Health (Grant number NS030549). G??bor Nyiri was supported by a J??nos Bolyai Research Scholarship. Andr??s Sz?nyi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of T??MOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program". Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, 1083, Hungary János Szentágothai Doctoral School of Neurosciences, Semmelweis University, Budapest, 1085, Hungary Cited By :19 Export Date: 28 May 2020 Correspondence Address: Nyiri, G.; Laboratory of Cerebral Cortex Research, Institute of Experimental Medicine, Hungarian Academy of SciencesHungary; email: nyiri.gabor@koki.mta.hu Chemicals/CAS: serotonin, 50-67-9; 4 aminobutyric acid, 28805-76-7, 56-12-2; Amino Acid Transport Systems, Acidic; gamma-Aminobutyric Acid; Serotonin; Transcription Factors; vesicular glutamate transporter 3, mouse Funding details: European Research Council, ERC, ERC-2011-ADG-294313 Funding details: National Institutes of Health, NIH, NS030549 Funding details: European Social Fund, ESF, A/1-11-1-2012-0001 Funding details: European Commission, EC Funding text 1: We thank Em?ke Sz??pn?? Simon, Katalin Lengyel and Katalin Iv??nyi for the excellent technical assistance. The authors wish to thank L??szl?? Barna, the Nikon Microscopy Center at IEM, Nikon Austria GmbH and Auro-Science Consulting Ltd for kindly providing technical support for fluorescent microscopy. This work was supported by the European Research Council (Grant number ERC-2011-ADG-294313, SERRACO) and the National Institutes of Health (Grant number NS030549). G??bor Nyiri was supported by a J??nos Bolyai Research Scholarship. Andr??s Sz?nyi was supported by the European Union and the State of Hungary, cofinanced by the European Social Fund in the framework of T??MOP 4.2.4. A/1-11-1-2012-0001 "National Excellence Program".
Subjects: R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában > R850-854 Experimental medicine / kisérleti orvostudomány
R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry / idegkórtan, neurológia, pszichiátria
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 28 Sep 2020 13:10
Last Modified: 28 Sep 2020 13:10
URI: http://real.mtak.hu/id/eprint/115117

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