Bősze, Zsuzsanna and Major, Péter and Baczkó, István and Odening, K. E. and Bodrogi, Lilla and Hiripi, László and Varró, András (2016) The potential impact of new generation transgenic methods on creating rabbit models of cardiac diseases. PROGRESS IN BIOPHYSICS AND MOLECULAR BIOLOGY, 121 (2). pp. 123-130. ISSN 0079-6107
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Abstract
Since the creation of the first transgenic rabbit thirty years ago, pronuclear microinjection remained the single applied method and resulted in numerous important rabbit models of human diseases, including cardiac deficiencies, albeit with low efficiency. For additive transgenesis a novel transposon mediated method, e.g., the Sleeping Beauty transgenesis, increased the efficiency, and its application to create cardiac disease models is expected in the near future. The targeted genome engineering nuclease family, e.g., the zink finger nuclease (ZFN), the transcription activator-like effector nuclease (TALEN) and the newest, clustered regularly interspaced short palindromic repeats (CRISPR) with the CRISPR associated effector protein (CAS), revolutionized the non-mouse transgenesis. The latest gene-targeting technology, the CRISPR/CAS system, was proven to be efficient in rabbit to create multi-gene knockout models. In the future, the number of tailor-made rabbit models produced with one of the above mentioned methods is expected to exponentially increase and to provide adequate models of heart diseases.
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| Additional Information: | \n Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, Gödöllő, H-2100, Hungary \n Department of Pharmacology & Pharmacotherapy, University of Szeged, Szeged, H-6720, Hungary \n Department of Cardiology and Angiology I, University Heart Center Freiburg, Freiburg, D-79106, Germany \n Cited By :7 \n Export Date: 29 November 2018 \n CODEN: PBIMA \n Correspondence Address: Bősze, Z.; Rabbit Genome and Biomodel Group, Animal Biotechnology Department of NARIC-Agricultural Biotechnology InstituteHungary; email: bosze@abc.hu \n Chemicals/CAS: DNA Transposable Elements Funding Agency and Grant Number: Hungarian Scientific Research Fund [OTKA CNK 77855, OTKA NK 104331, OTKA NN 110896, OTKA NK 104397]; RGB-Net COST Action [TD1101]; SALAAM COST Action [BM1308] Funding text: This work was supported by the Hungarian Scientific Research Fund (OTKA CNK 77855 and OTKA NK 104331 to A.V., OTKA NN 110896 to I.B., OTKA NK 104397 to Z.B) and by the RGB-Net COST Action TD1101 and SALAAM COST Action BM1308. Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, Gödöllő, H-2100, Hungary Department of Pharmacology & Pharmacotherapy, University of Szeged, Szeged, H-6720, Hungary Department of Cardiology and Angiology I, University Heart Center Freiburg, Freiburg, D-79106, Germany Cited By :9 Export Date: 9 July 2020 CODEN: PBIMA Correspondence Address: Bősze, Z.; Rabbit Genome and Biomodel Group, Animal Biotechnology Department of NARIC-Agricultural Biotechnology InstituteHungary; email: bosze@abc.hu Chemicals/CAS: DNA Transposable Elements Funding Agency and Grant Number: Hungarian Scientific Research FundOrszagos Tudomanyos Kutatasi Alapprogramok (OTKA) [OTKA CNK 77855, OTKA NK 104331, OTKA NN 110896, OTKA NK 104397]; RGB-Net COST Action [TD1101]; SALAAM COST Action [BM1308] Funding text: This work was supported by the Hungarian Scientific Research Fund (OTKA CNK 77855 and OTKA NK 104331 to A.V., OTKA NN 110896 to I.B., OTKA NK 104397 to Z.B) and by the RGB-Net COST Action TD1101 and SALAAM COST Action BM1308. Rabbit Genome and Biomodel Group, NARIC-Agricultural Biotechnology Institute, Gödöllő, H-2100, Hungary Department of Pharmacology & Pharmacotherapy, University of Szeged, Szeged, H-6720, Hungary Department of Cardiology and Angiology I, University Heart Center Freiburg, Freiburg, D-79106, Germany Cited By :10 Export Date: 1 December 2020 CODEN: PBIMA Correspondence Address: Bősze, Z.; Rabbit Genome and Biomodel Group, Animal Biotechnology Department of NARIC-Agricultural Biotechnology InstituteHungary; email: bosze@abc.hu |
| Uncontrolled Keywords: | Animals; Humans; RABBITS; GENETICS; GENOMICS; GENOMICS; Heart Diseases; human; heart disease; animal; Animals, Genetically Modified; Disease Models, Animal; Rabbit; disease model; transgenic animal; DNA Transposable Elements; transposon; transposon; Knockout; Gene Transfer Techniques; gene transfer; transgenic rabbit; sleeping beauty transposon; Designer nucleases; Knock-in; |
| Subjects: | R Medicine / orvostudomány > RC Internal medicine / belgyógyászat |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 07 Jan 2021 15:11 |
| Last Modified: | 07 Jan 2021 15:11 |
| URI: | http://real.mtak.hu/id/eprint/119296 |
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