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A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression

Torras-Llort, Mònica and Medina-Giró, Sònia and Escudero-Ferruz, Paula and Lipinszki, Zoltán and Moreno-Moreno, Olga and Kármán, Zoltán (2020) A fraction of barrier-to-autointegration factor (BAF) associates with centromeres and controls mitosis progression. COMMUNICATIONS BIOLOGY, 3 (1). ISSN 2399-3642

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Abstract

Barrier-to-Autointegration Factor (BAF) is a conserved nuclear envelope (NE) component that binds chromatin and helps its anchoring to the NE. Cycles of phosphorylation and dephosphorylation control BAF function. Entering mitosis, phosphorylation releases BAF from chromatin and facilitates NE-disassembly. At mitotic exit, PP2A-mediated dephosphorylation restores chromatin binding and nucleates NE-reassembly. Here, we show that in Drosophila a small fraction of BAF (cenBAF) associates with centromeres. We also find that PP4 phosphatase, which is recruited to centromeres by CENP-C, prevents phosphorylation and release of cenBAF during mitosis. cenBAF is necessary for proper centromere assembly and accurate chromosome segregation, being critical for mitosis progression. Disrupting cenBAF localization prevents PP2A inactivation in mitosis compromising global BAF phosphorylation, which in turn leads to its persistent association with chromatin, delays anaphase onset and causes NE defects. These results suggest that, together with PP4 and CENP-C, cenBAF forms a centromere-based mechanism that controls chromosome segregation and mitosis progression. © 2020, The Author(s).

Item Type: Article
Additional Information: Institute of Molecular Biology of Barcelona, CSIC, Barcelona, Spain Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain MTA SZBK Lendület Laboratory of Cell Cycle Regulation and Institute of Biochemistry, Biological Research Centre, Szeged, Hungary Doctoral School of Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary School of Biological Sciences, Institute for Life Sciences, University of Southampton, Southampton, SO17 1BJ, United Kingdom Export Date: 27 August 2020 Correspondence Address: Torras-Llort, M.; Institute of Molecular Biology of Barcelona, CSIC, Institute of Molecular Biology of Barcelona, CSICSpain; email: mtlbmc@ibmb.csic.es
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 29 Jan 2021 10:59
Last Modified: 29 Jan 2021 10:59
URI: http://real.mtak.hu/id/eprint/120219

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