Wirth, Roland and Maróti, Gergely and Mihók, Róbert and Simon-Fiala, Donát and Antal, Márk Ádám and Pap, Bernadett and Nagy-Demcsák, Anett and Minárovits, János and Kovács, Kornél Lajos (2020) A case study of salivary microbiome in smokers and non-smokers in Hungary: analysis by shotgun metagenome sequencing. JOURNAL OF ORAL MICROBIOLOGY, 12 (1). ISSN 2000-2297
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Abstract
Objective To investigate the role of cigarette smoking in disease-development through altering the composition of the oral microbial community. Periodontitis and oral cancer are highly prevalent in Hungary; therefore, the salivary microbiome of smoker and non-smoker Hungarian adults was characterized. Methods Shotgun metagenome sequencing of salivary DNA samples from 22 individuals (11 non-smokers and 11 current smokers) was performed using the Ion Torrent PGMTM platform. Quality-filtered reads were analysed by both alignment-based sequence similarity searches and genome-centric binning. Results Prevotella, Veillonella and Streptococcus were the predominant genera in the saliva of both groups. Although the overall composition and diversity of the microbiota were similar, Prevotella was significantly more abundant in salivary samples of current smokers compared to non-smokers. Members of the genus Prevotella were implicated in the development of inflammatory diseases and oral cancer. The abundance of the genus Megasphaera also increased in current smokers, whereas the genera Neisseria, Oribacterium, Capnocytophaga and Porphyromonas were significantly reduced. The data generated by read-based taxonomic classification and genome-centric binning mutually validated the two distinct metagenomic approaches. Conclusion Smoking-associated dysbiosis of the salivary microbiome in current cigarette smokers, especially increased abundance of Prevotella and Megasphaera genera, may facilitate disease development.
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| Additional Information: | Department of Biotechnology, University of Szeged, Szeged, Hungary Institute of Plant Biology, Biological Research Center, Szeged, Hungary Department of Operative and Esthetic Dentistry, Faculty of Dentistry, University of Szeged, Szeged, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry,, University of Szeged, Szeged, Hungary Export Date: 28 August 2020 Correspondence Address: Kovács, K.L.; Department of Biotechnology, University of SzegedHungary; email: kovacs.kornel@bio.u-szeged.hu Chemicals/CAS: carbon monoxide, 630-08-0; DNA, 9007-49-2 Funding details: Magyar Tudományos Akadémia, MTA Funding details: European Regional Development Fund, FEDER Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI, PD121085, FK123899 Funding text 1: The support of the grant GINOP-2.3.2-15-2016-00011 by the European Regional Development Fund to a project led by JM is kindly acknowledged. Additional projects GINOP-2.2.1-15-2017-00081, GINOP-2.2.1-15-2017-00033, and EFOP-3.6.2-16-2017-00010 also contributed to this work. The grants were managed by European Union Economic Development and Innovation Operational Programme, National Research, Development and Innovation Office (NKFIH), Hungary. RW and GM received support from the Hungarian NKFIH fund projects PD121085 and FK123899 financed under the PD16 and FK16 funding schemes. This work was also supported by the János Bolyai Research Scholarship (for GM) of the Hungarian Academy of Sciences. Department of Biotechnology, University of Szeged, Szeged, Hungary Institute of Plant Biology, Biological Research Center, Szeged, Hungary Department of Operative and Esthetic Dentistry, Faculty of Dentistry, University of Szeged, Szeged, Hungary Department of Oral Biology and Experimental Dental Research, Faculty of Dentistry,, University of Szeged, Szeged, Hungary Export Date: 19 September 2020 Correspondence Address: Kovács, K.L.; Department of Biotechnology, University of SzegedHungary; email: kovacs.kornel@bio.u-szeged.hu Chemicals/CAS: carbon monoxide, 630-08-0; DNA, 9007-49-2 Funding details: Magyar Tudományos Akadémia, MTA Funding details: European Regional Development Fund, FEDER Funding details: Nemzeti Kutatási Fejlesztési és Innovációs Hivatal, NKFI, PD121085, FK123899 Funding text 1: The support of the grant GINOP-2.3.2-15-2016-00011 by the European Regional Development Fund to a project led by JM is kindly acknowledged. Additional projects GINOP-2.2.1-15-2017-00081, GINOP-2.2.1-15-2017-00033, and EFOP-3.6.2-16-2017-00010 also contributed to this work. The grants were managed by European Union Economic Development and Innovation Operational Programme, National Research, Development and Innovation Office (NKFIH), Hungary. RW and GM received support from the Hungarian NKFIH fund projects PD121085 and FK123899 financed under the PD16 and FK16 funding schemes. This work was also supported by the János Bolyai Research Scholarship (for GM) of the Hungarian Academy of Sciences. |
| Uncontrolled Keywords: | *Smoking; Prevotella; oral cancer.; saliva metagenome; read-based taxonomy; genome-centric binning; Megasphaera; |
| Subjects: | Q Science / természettudomány > QR Microbiology / mikrobiológia |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 29 Jan 2021 13:10 |
| Last Modified: | 29 Jan 2021 13:10 |
| URI: | http://real.mtak.hu/id/eprint/120244 |
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