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Biomolecule composition and draft genome of a novel, high-lipid producing Scenedesmaceae microalga

Soós, Vilmos and Shetty, Prateek and Maróti, Gergely and Incze, Norbert and Badics, Eszter and Balázs, Ervin (2021) Biomolecule composition and draft genome of a novel, high-lipid producing Scenedesmaceae microalga. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 54. ISSN 2211-9264

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Abstract

Lipid biosynthesis in microalgae can be stimulated by cultivation in low nitrogen medium. MACC-401 was isolated from the soil surface in Tres Marias (MG-Brazil). The strain shows the morphological characteristics of the Scenedesmaceae green algae. The daily biomass and lipid production of MACC-401 is remarkable, 0.36 g L−1 and 110 mg L−1, respectively. Exploration of the genetic background of this promising strain not only allows the utilization of this species for industrial-scale lipid production, but also provides genetic targets to select lipid-producing strains from microalgae collections. We conducted physiological experiments by cultivating MACC-401 in complete and N-limited media and performed genome sequencing as well as transcriptome analysis. The estimated nuclear genome size of MACC-401 is 99.503 Mbp and the chloroplast genome is 0.15 Mbp. The phylogenetic analysis confirmed that the MACC-401 belongs to the Scenedesmaceae family, and represents a genetically distinct accession in this family. A basic comparative transcriptome analysis resulted in the identification of N-starvation responsive genes, which could serve as markers to monitor the onset of lipid accumulation in algal cultures.

Item Type: Article
Uncontrolled Keywords: Transcriptome; genome sequencing; lipid accumulation; Scenedesmaceae; N-starvation;
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 02 Sep 2021 05:19
Last Modified: 02 Sep 2021 05:19
URI: http://real.mtak.hu/id/eprint/128965

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