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Aggregation-related quenching of LHCII fluorescence in liposomes revealed by single-molecule spectroscopy

Tutkus, Marijonas and Chmeliov, Jevgenij and Trinkunas, Gediminas and Akhtar, Parveen and Lambrev, Petar H. and Valkunas, Leonas (2021) Aggregation-related quenching of LHCII fluorescence in liposomes revealed by single-molecule spectroscopy. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 218. ISSN 1011-1344

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Abstract

Incorporation of membrane proteins into reconstituted lipid membranes is a common approach for studying their structure and function relationship in a native-like environment. In this work, we investigated fluorescence properties of liposome-reconstituted major light-harvesting complexes of plants (LHCII). By utilizing liposome labelling with the fluorescent dye molecules and single-molecule microscopy techniques, we were able to study truly liposome-reconstituted LHCII and compare them with bulk measurements and liposome-free LHCII aggregates bound to the surface. Our results showed that fluorescence lifetime obtained in bulk and in single liposome measurements were correlated. The fluorescence lifetimes of LHCII were shorter for liposome-free LHCII than for reconstituted LHCII. In the case of liposome-reconstituted LHCII, fluorescence lifetime showed dependence on the protein density reminiscent to concentration quenching. The dependence of fluorescence lifetime of LHCII on the liposome size was not significant. Our results demonstrated that fluorescence quenching can be induced by LHCII – LHCII interactions in reconstituted membranes, most likely occurring via the same mechanism as photoprotective non-photochemical quenching in vivo. © 2021 Elsevier B.V.

Item Type: Article
Uncontrolled Keywords: AGGREGATION; FLUORESCENCE; Liposomes; SINGLE-MOLECULE; confocal microscopy; non-photochemical quenching; Fluorescence lifetime imaging; surface immobilization; Light-harvesting complex II; Liposome-reconstitution; Protein density;
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia
Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 02 Sep 2021 07:36
Last Modified: 02 Sep 2021 07:36
URI: http://real.mtak.hu/id/eprint/128971

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