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Identification of Ovine-Associated Staphylococci by MALDI-TOF Mass Spectrometry

Tonamo, A. and Komlósi, I. and Varga, L. and Kačániová, M. and Peles, F. (2021) Identification of Ovine-Associated Staphylococci by MALDI-TOF Mass Spectrometry. ACTA ALIMENTARIA: AN INTERNATIONAL JOURNAL OF FOOD SCIENCE, 50 (2). pp. 210-218. ISSN 0139-3006 (print); 1588-2535 (online)

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Abstract

The objective of this study was to use matrix-assisted laser desorption ionisation–time of flight mass spectrometry (MALDI-TOF MS) for the identification of ovine-associated staphylococci. Presumptive Staphylococcus isolates were recovered from ovine udder surface (US), individual raw milk, bulk tank milk, and cheese samples and were characterised by conventional phenotypic methods. A total of 69 bacterial isolates were further confirmed by MALDI-TOF MS. Forty-two (60.9%) of 69 isolates were successfully identified on genus and species level. Two thirds (n=28) of the 42 identified isolates were shown to be Staphylococcus spp. These 28 staphylococcal isolates formed two clusters, one consisting of 22 Staphylococcus aureus strains and the other composed of 6 non-aureus staphylococci, including S. simulans (n=3), S. auricularis, S. equorum, and S. haemolyticus. MALDI-TOF MS has proven to be a reliable tool for the identification of staphylococci from raw sheep’s milk, especially bulk tank milk; however, currently it appears to be less useful for the identification of bacterial isolates originating from ovine US samples. This is the first study to evaluate the applicability of MALDI-TOF MS for identification of Staphylococcus spp. in ovine raw milk, cheese, and US samples in Hungary.

Item Type: Article
Additional Information: MTA KFB támogatási szerződés alapján archiválva
Uncontrolled Keywords: MALDI-TOF MS; Staphylococcus; coagulase; sheep milk; cheese
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 06 Sep 2021 14:44
Last Modified: 06 Sep 2021 14:44
URI: http://real.mtak.hu/id/eprint/129070

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