Molecular characterization of ESBL- producing uropathogenic Escherichia coli recovered from urine samples of patients attending a University Teaching hospital in Nigeria

Adekanmbi, Abimbola Olumide and Usidamen, Sandra and Onilude, Anthony Abiodun (2021) Molecular characterization of ESBL- producing uropathogenic Escherichia coli recovered from urine samples of patients attending a University Teaching hospital in Nigeria. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 68 (3). pp. 169-176. ISSN 1217-8950

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Abstract

Infection of the urinary tract ranks as one of the most common infections affecting people worldwide and its treatment is made complicated by the rising incidence of antibiotic resistance. This study aimed to detect extended spectrum beta-lactamase (ESBL) genes and antibiotic resistance profile of uropathogenic Escherichia coli (E. coli) recovered from patients attending a University Teaching hospital in Nigeria. Uropathogenic E. coli isolates were obtained from the culture collection of Department of Microbiology and Parasitology of the University Teaching hospital for a period of four months (October 2019-January, 2020). Antibiotic susceptibility testing was done using the disc diffusion method while phenotypic ESBL production was detected using double disc synergy test (DDST). Detection of beta-lactamase genes was done using Real-Time PCR. Forty-nine E. coil isolates were recovered from 120 urine samples, with 24 (49%) being ESBL, positive. The resistance to antibiotics in the ESBL, producers was: ciprofloxacin (100%), cefotaxime (100%), cefpodoxime (100%), tetracycline (95.7%), ceftazidime (56.7%), amoxicillin-clavulanate (50%), gentamicin (33.3%), and imipenem (0%). All the ESBL producers carried bla(TEM), bla(CTX-M-1) and bla(CTX-M-9), 75% (18/24) carried bla(SHV), while bla(CTX-M-2), bia(CTX-M-8) and bla(CTX-M-25) groups were detected in 20.8% (5/24) of the isolates. There was co-occurrence of CTX-M, SHV and TEM beta-lactamases in 79.2% (19/24) isolates, while five isolates (20.8%) co-harbored bla(CTX-M) m and bia(TEM). This study showed a high level of multidrug resistance and ESBL gene carriage in uropathogenic E. coli obtained in this study, suggesting a likely review of therapeutic options in the treatment of UTI to clamp down on the rising cases of antibiotic resistance.

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