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Linezolid resistance among multidrug-resistant Mycobacterium tuberculosis clinical isolates in Iran

Khosravi, Azar Dokht and Tabandeh, Mohammad Reza and Shahi, Fatemeh and Salmanzadeh, Shokrollah (2021) Linezolid resistance among multidrug-resistant Mycobacterium tuberculosis clinical isolates in Iran. ACTA MICROBIOLOGICA ET IMMUNOLOGICA HUNGARICA, 68 (3). pp. 203-207. ISSN 1217-8950

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Abstract

The management of multidrug-resistant (MDR) and extensively drug-resistant tuberculosis (XDR-TB) presents a main challenge and the drug options for treating these infections are very limited. Linezolid (LNZ) has recently been approved for the treatment of MDR and XDR-TB. But, there are narrow data on genotypic and phenotypic LNZ resistance in clinical isolates. So, we aimed to determine the prevalence of LNZ resistance and to identify the mutations associated with LNZ resistance among clinical MDR-TB isolates. The minimum inhibitory concentration (MIC) values of LNZ, for 22 MDR-TB isolates were determined by broth microdilution method. All MDR-TB isolates were sequenced in the rrl and rp1C genes conferring LNZ, resistance. LNZ, resistance was found in 3 (13.6%) of 22 MDR-TB isolates. The MICs of LNZ were 8 mu g/mL for two isolates and 16 mu g/mL for one isolate. The 421 (A/G) and 449 (T/A) mutations in rplC gene were detected in one of the LNZ-resistant isolates. There was no mutation in rrl gene. The results reveal that the prevalence of LNZ-resistant isolates is 13.6% among MDR-TB isolates and drug susceptibility testing (DST) against LNZ is useful in the management of complicated and drug-resistant cases. However, further studies could identify other possible genetic mechanism of resistance in TB.

Item Type: Article
Additional Information: Funding Agency and Grant Number: Research Affairs, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran [OG-9736]
Uncontrolled Keywords: MUTATION; MUTATIONS; immunology; multidrug resistance; Mycobacterium tuberculosis; linezolid; extensively drug-resistant;
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 18 Oct 2021 07:39
Last Modified: 30 Sep 2022 23:15
URI: http://real.mtak.hu/id/eprint/132623

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