REAL

Mannose-binding lectin level and deficiency is not associated with inflammatory bowel diseases, disease phenotype, serology profile, and NOD2/CARD15 genotype in a large Hungarian cohort

Papp, Mária and Lakatos, Péter László and Hársfalvi, Jolán and Farkas, G. and Palatka, Károly and Udvardy, Miklós and Molnár, Tamás and Farkas, Klaudia and Nagy, Ferenc and Veres, Gábor and Lakatos, László and Kocsis, Ágnes Katalin and Papp, János Mihály and Altorjay, István (2010) Mannose-binding lectin level and deficiency is not associated with inflammatory bowel diseases, disease phenotype, serology profile, and NOD2/CARD15 genotype in a large Hungarian cohort. HUMAN IMMUNOLOGY, 71 (4). pp. 407-413. ISSN 0198-8859

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Abstract

Mannose-binding lectin (MBL) is a major, soluble, pattern-recognition molecule and an important component of the innate host defense. The role of MBL in inflammatory bowel diseases (IBDs) is controversial. We determined the prevalence of MBL deficiency in a Hungarian IBD patients' cohort, and whether it is associated with the antimicrobial antibody formation or particular clinical manifestations. Nine hundred ninety IBD patients and 225 healthy subjects were investigated. Sera were assayed for MBL and a panel of antimicrobial antibodies (anti-OMP, anti-Saccharomyces cerevisiae antibodies, and antiglycans) by ELISA. TLR4 and NOD2/CARD15 variants were tested by polymerase chain reaction/restriction fragment length polymorphism. Median MBL level was not significantly different between IBDs (Crohn's disease [CD]: 929; ulcerative colitis [UC]: 810 ng/ml) and the control group (1027 ng/ml), as well as the prevalence of absolute MBL deficiency (<100 ng/ml) (CD: 15.0%, UC: 18.4%, controls: 15.6%). The presence of a low MBL level (<500 ng/ml) was not associated with any of the examined serologic markers, or their combinations. In addition, there was no association with the clinical presentation, disease course, or response to treatment. TLR4 variant genotype was more common in CD patients without MBL deficiency (11% vs. 1.7%, OR: 7.29, 95% CI: 1.08-53.9, p = 0.02). We failed to confirm any association between MBL deficiency and serologic marker positivity. MBL deficiency was not predictive for clinical phenotype or disease activity in IBDs.

Item Type: Article
Additional Information: Maria Papp and Peter Laszlo Lakatos contributed equally to the work and both should be considered as first authors. Multicentrikus tanulmány further Hungarian IBD Study Group members are: Semmelweis University, 1st Department of Medicine, Budapest: Simon Fischer, Peter Fuszek, Orsolya Gemela, Tamas Szamosi, Peter Vargha; Csolnoky F. County Hospital, 1st Department of Medicine, Veszprém: Zsuzsanna Erdelyi, Gabor Mester, Csaba Molnar, Tunde Pandur; University of Szeged, 1st Department of Medicine, Szeged: Ferenc Nagy, Janos Lonovics; Department of Gastroenterology and Surgery, St. Margit Hospital, Budapest: Levente Balint, Pal Demeter, Istvan Dobo, Ferenc Huoranszky; Semmelweis University, 2nd Department of Medicine, Semmelweis University, Budapest: Laszlo Herszenyi, Pal Miheller, Zsolt Tulassay
Uncontrolled Keywords: Toll-Like Receptor 4; Saccharomyces cerevisiae Proteins; Polymorphism, Genetic; Nod2 Signaling Adaptor Protein; Mitochondrial Membrane Transport Proteins; Middle Aged; Humans; Genetic Predisposition to Disease; Genetic Association Studies; DNA Mutational Analysis; Biological Markers; Antibodies, Fungal; ulcerative colitis; Serology; restriction fragment length polymorphism; priority journal; PREVALENCE; polymerase chain reaction; PHENOTYPE; mannose binding lectin deficiency; Male; major clinical study; immune deficiency; Hungary; human; Genotype; Female; enzyme linked immunosorbent assay; enteritis; disease course; disease association; disease activity; Crohn disease; controlled study; cohort analysis; ARTICLE; antibody production; Adult; unclassified drug; toll like receptor 4; Saccharomyces cerevisiae antibody; outer membrane protein antibody; mannose binding lectin; glycan antibody; fungus antibody; caspase recruitment domain protein 15; C reactive protein; TLR4; MANNOSE-BINDING LECTIN; Inflammatory Bowel Diseases; Antimicrobial antibodies
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Q Science / természettudomány > QR Microbiology / mikrobiológia > QR180 Immunology / immunológia
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 08 Jul 2014 05:49
Last Modified: 08 Jul 2014 05:49
URI: http://real.mtak.hu/id/eprint/13562

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