Rare Earth Element Sequestration by Aspergillus oryzae Biomass

Boczonádi, Imre and Jakab, Ágnes and Baranyai, Edina and Tóth, Csilla Noémi and Daróczi, Lajos and Csernoch, László and Kis, Gréta and Antal, Miklós and Pusztahelyi, Tünde and Emri, Tamás and Fábián, István and Pócsi, István (2021) Rare Earth Element Sequestration by Aspergillus oryzae Biomass. ENVIRONMENTAL TECHNOLOGY, 42 (24). pp. 3725-3735. ISSN 0959-3330 (print); 1479-487X (online)

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Abstract

The fungus Aspergillus oryzae could be shown to be a viable alternative for biosorption of valuable metals from solution. Fungal biomass can be obtained easily in high quantities as a waste of biofermentation processes, and used in a complex, multi-phase solution mimicking naturally occurring, mining-affected water samples. With test solution formulated after natural conditions, formation of secondary Al and Fe phases co-precipitating Ce was recorded in addition to specific biosorption of rare earth elements. Remarkably, the latter were removed from the solution despite the presence of high concentrations of interfering Fe and Al. The biomass was viable even after prolonged incubation in the metal solution, and minimal inhibitory concentrations for single metals were higher than those in the test solution. While precipitation/biosorption of Ce (maximal biosorption efficiency was 58.0 ± 22.3% after 6 h of incubation) coincided with the gross removal of Fe from the metal solution, Y (81.5 ± 11.3% efficiency, 24 h incubation) and Nd (87.4 ± 9.1% efficiency, 24 h incubation) were sequestered later, similarly to Ni and Zn. The biphasic binding pattern specific to single metals could be connected to dynamically changing pH and NH4+ concentrations, which were attributed to the physiological changes taking place in starving A. oryzae biomass. The metals were found extracellularly in minerals associated with the cell wall, and intracellularly precipitated in the vacuoles. The latter process was explained with intracellular metal detoxification resulting in metal resistance.

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