Gasperl, A. and Balogh, Eszter and Boldizsár, Ákos and Kemeter, N. and Pirklbauer, R. and Kalapos, Balázs and Szalai, Gabriella and Kocsy, Gábor (2021) Comparison of light condition-dependent differences in the accumulation and subcellular localization of glutathione in arabidopsis and wheat. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22 (2). ISSN 1661-6596
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Abstract
This study aimed to clarify whether the light condition-dependent changes in the redox state and subcellular distribution of glutathione were similar in the dicotyledonous model plant Arabidopsis (wild-type, ascorbate-and glutathione-deficient mutants) and the monocotyledonous crop species wheat (Chinese Spring variety). With increasing light intensity, the amount of its reduced (GSH) and oxidized (GSSG) form and the GSSG/GSH ratio increased in the leaf extracts of both species including all genotypes, while far-red light increased these parameters only in wheat except for GSH in the GSH-deficient Arabidopsis mutant. Based on the expression changes of the glutathione metabolism-related genes, light intensity influences the size and redox state of the glu-tathione pool at the transcriptional level in wheat but not in Arabidopsis. In line with the results in leaf extracts, a similar inducing effect of both light intensity and far-red light was found on the total glutathione content at the subcellular level in wheat. In contrast to the leaf extracts, the inducing influence of light intensity on glutathione level was only found in the cell compartments of the GSH-deficient Arabidopsis mutant, and far-red light increased it in both mutants. The observed general and genotype-specific, light-dependent changes in the accumulation and subcellular distribution of glutathione participate in adjusting the redox-dependent metabolism to the actual environmental conditions. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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| Additional Information: | Institute of Biology, Plant Sciences, University of Graz, NAWI Graz, Graz, 8010, Austria Agricultural Institute, ELKH Centre for Agricultural Research, Martonvásár, 2462, Hungary Export Date: 18 January 2021 Correspondence Address: Kocsy, G.; Agricultural Institute, ELKH Centre for Agricultural ResearchHungary; email: kocsy.gabor@atk.hu Funding details: Austrian Science Fund, FWF, I 2818, HU-0616 Funding details: Austrian Science Fund, FWF Funding details: National Research, Development and Innovation Office, TéT_15-1-2016-0048, ANN117949, K131638 Funding details: Austrian Science Fund, FWF Funding details: Austrian Science Fund, FWF Funding text 1: Funding: This research was supported by the National Research, Development and Innovation Office (grants ANN117949, K131638, TéT_15-1-2016-0048 to G.K.), by the Austrian Science Fund (FWF, I 2818 to M.M) and the OeAD (Österreich-Ungarn HU-0616 to M.M. and G.K.). Funding text 2: Acknowledgments The authors wish to thank Apollónia Horváth and Mónika Fehér for their help in plant cultivation and sampling and Karina Hauer for her help with sample preparation for in situ glutathione content analysis. Open Access Funding by the Austrian Science Fund (FWF). Institute of Biology, Plant Sciences, University of Graz, NAWI Graz, Graz, 8010, Austria Agricultural Institute, ELKH Centre for Agricultural Research, Martonvásár, 2462, Hungary Export Date: 1 February 2021 Correspondence Address: Kocsy, G.; Agricultural Institute, Hungary; email: kocsy.gabor@atk.hu Funding details: Austrian Science Fund, FWF, I 2818, HU-0616 Funding details: Austrian Science Fund, FWF Funding details: National Research, Development and Innovation Office, TéT_15-1-2016-0048, ANN117949, K131638 Funding details: Austrian Science Fund, FWF Funding details: Austrian Science Fund, FWF Funding text 1: Funding: This research was supported by the National Research, Development and Innovation Office (grants ANN117949, K131638, TéT_15-1-2016-0048 to G.K.), by the Austrian Science Fund (FWF, I 2818 to M.M) and the OeAD (Österreich-Ungarn HU-0616 to M.M. and G.K.). Funding text 2: Acknowledgments The authors wish to thank Apollónia Horváth and Mónika Fehér for their help in plant cultivation and sampling and Karina Hauer for her help with sample preparation for in situ glutathione content analysis. Open Access Funding by the Austrian Science Fund (FWF). Institute of Biology, Plant Sciences, University of Graz, NAWI Graz, Graz, 8010, Austria Agricultural Institute, ELKH Centre for Agricultural Research, Martonvásár, 2462, Hungary Export Date: 2 February 2021 Correspondence Address: Kocsy, G.; Agricultural Institute, Hungary; email: kocsy.gabor@atk.hu Funding details: Austrian Science Fund, FWF, I 2818, HU-0616 Funding details: Austrian Science Fund, FWF Funding details: National Research, Development and Innovation Office, TéT_15-1-2016-0048, ANN117949, K131638 Funding details: Austrian Science Fund, FWF Funding details: Austrian Science Fund, FWF Funding text 1: Funding: This research was supported by the National Research, Development and Innovation Office (grants ANN117949, K131638, TéT_15-1-2016-0048 to G.K.), by the Austrian Science Fund (FWF, I 2818 to M.M) and the OeAD (Österreich-Ungarn HU-0616 to M.M. and G.K.). Funding text 2: Acknowledgments The authors wish to thank Apollónia Horváth and Mónika Fehér for their help in plant cultivation and sampling and Karina Hauer for her help with sample preparation for in situ glutathione content analysis. Open Access Funding by the Austrian Science Fund (FWF). Funding Agency and Grant Number: National Research, Development and Innovation OfficeNational Research, Development & Innovation Office (NRDIO) - Hungary [ANN117949, K131638, TeT_15-1-2016-0048]; Austrian Science Fund (FWF)Austrian Science Fund (FWF) [I 2818]; OeAD (Osterreich-Ungarn) [HU-0616] Funding text: This research was supported by the National Research, Development and Innovation Office (grants ANN117949, K131638, TeT_15-1-2016-0048 to G.K.), by the Austrian Science Fund (FWF, I 2818 to M.M) and the OeAD (Osterreich-Ungarn HU-0616 to M.M. and G.K.). |
| Uncontrolled Keywords: | WHEAT; Light intensity; GLUTATHIONE; ARABIDOPSIS; REDOX STATE; far-red light; cell compartment; |
| Subjects: | Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia Q Science / természettudomány > QK Botany / növénytan |
| SWORD Depositor: | MTMT SWORD |
| Depositing User: | MTMT SWORD |
| Date Deposited: | 29 Mar 2022 16:44 |
| Last Modified: | 29 Mar 2022 16:44 |
| URI: | http://real.mtak.hu/id/eprint/139894 |
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