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Rac1 Participates in Thermally Induced Alterations of the Cytoskeleton, Cell Morphology and Lipid Rafts, and Regulates the Expression of Heat Shock Proteins in B16F10 Melanoma Cells

Güngör, Burçin and Gombos, Imre and Crul, Tim and Ayaydin, Ferhan and Mátés, Lajos and Török, Zsolt and Szabó, László and Vigh, László and Horváth, Ibolya (2014) Rac1 Participates in Thermally Induced Alterations of the Cytoskeleton, Cell Morphology and Lipid Rafts, and Regulates the Expression of Heat Shock Proteins in B16F10 Melanoma Cells. PLOS ONE, 9 (2). e89136. ISSN 1932-6203

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Abstract

Eukaryotic cells exhibit a characteristic response to hyperthermic treatments involving morphological and cytoskeletetal alterations and induction of heat shock protein synthesis. Small GTPases of the Ras superfamily are known to serve as molecular switches which mediate responses to extracellular stimuli. Here we addressed how small GTPase Rac1 integrates signals from heat stress and induces simultaneously various cellular changes in mammalian cells. Evidencing that Rac1 is implicated in the heat shock response, first we showed that both mild (41.5 °C) and severe (43 °C) heat shock induced membrane translocation of Rac1. Upon the inhibition of the activation (NSC23766) or palmitoylation (2-bromopalmitate) of Rac1, the size of its plasmamembrane bound pool was significantly decreased. Heat shock induced alterations in the cytoskeleton and cell morphology were prevented using the above inhibitors. Earlier we hypothesized that the level and size distribution pattern of Chol-rich rafts are directly coupled to the triggering mechanism of stress sensing and signaling. As a striking finding, when plasma membrane localization of Rac1 was inhibited we obtained reduced raft domain size at 43 °C. The above documented effects of Rac1 inhibitors were accompanied with a strongly decreased expression of hsp25 and hsp70 under both mild and severe heat stress conditions.

Item Type: Article
Uncontrolled Keywords: Rac1, heat stress protein, membrane localization, actin cytoskeleton, cell morphology, rafts, heat sensing and signaling
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Q Science / természettudomány > QD Chemistry / kémia
Depositing User: Dr. xAranka xPilbáth
Date Deposited: 08 Sep 2014 07:40
Last Modified: 08 Sep 2014 07:40
URI: http://real.mtak.hu/id/eprint/14460

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