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Metatranscriptomic Analyses Reveal the Functional Role of Botrytis cinerea in Biochemical and Textural Changes during Noble Rot of Grapevines

Hegyi, Ádám István and Otto, Margot and Geml, József and Hegyi-Kaló, Júlia and Kun, József and Gyenesei, Attila and Pierneef, Rian and Váczy, Kálmán Zoltán (2022) Metatranscriptomic Analyses Reveal the Functional Role of Botrytis cinerea in Biochemical and Textural Changes during Noble Rot of Grapevines. JOURNAL OF FUNGI, 8 (4). p. 378. ISSN 2309-608X

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Abstract

Botrytis cinerea, can lead to the formation of noble rot (NR) of grape berries under certain environmental conditions, resulting in favored metabolic and physical changes necessary for producing highly regarded botrytized wines. The functional genes involved in the textural and biochemical processes are still poorly characterized. We generated and analyzed metatranscriptomic data from healthy (H) berries and from berries representing the four stages of NR from the Tokaj wine region in Hungary over three months. A weighted gene co-expression network analysis (WGCNA) was conducted to link B. cinerea functional genes to grape berry physical parameters berry hardness (BH), berry skin break force (F_sk), berry skin elasticity (E_sk), and the skin break energy (W_sk). Clustered modules showed that genes involved in carbohydrate and protein metabolism were significantly enriched in NR, highlighting their importance in the grape berry structural integrity. Carbohydrate active enzymes were particularly up-regulated at the onset of NR (during the transition from phase I to II) suggesting that the major structural changes occur early in the NR process. In addition, we identified genes expressed throughout the NR process belonging to enriched pathways that allow B. cinerea to dominate and proliferate during this state, including sulphate metabolizing genes and genes involved in the synthesis of antimicrobials.

Item Type: Article
Uncontrolled Keywords: otrytis cinerea; WGCNA clustering; differential expression; enrichment; noble rot; secondary metabolites
Subjects: Q Science / természettudomány > QK Botany / növénytan
Depositing User: Tibor Gál
Date Deposited: 12 Sep 2022 06:54
Last Modified: 03 Apr 2023 07:58
URI: http://real.mtak.hu/id/eprint/148267

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