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Inflammasome activation in end-stage heart failure-associated atrial fibrillation

Kugler, Szilvia and Onódi, Zsófia and Ruppert, Mihály and Sayour, Alex Ali and Oláh, Attila and Benke, Kálmán and Ferdinandy, Péter and Merkely, Béla and Radovits, Tamás and Varga, Zoltán V. (2022) Inflammasome activation in end-stage heart failure-associated atrial fibrillation. ESC Heart Failure, 9. pp. 2747-2752. ISSN 2055-5822

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Abstract

Aims Inflammatory pathways are increasingly recognized as an important factor in the pathophysiology of both heart failure (HF) and atrial fibrillation (AF). However, there is no data about inflammation-related histological and molecular alterations in HF-associated AF. The objective of our study was to investigate inflammatory pathways and fibrosis in end-stage HF- associated AF. Methods and results Left atrial samples of 24 male patients with end stage ischemic HF undergoing heart transplantation were analysed. Twelve patients suffered from sustained AF while the others had no documented AF. The expression of inflam- masome sensors and their downstream signalling were investigated by Western blot. No differences were observed in the ex- pression of inflammasome sensors between the two groups, while cleaved caspase-1 increased tendentiously in the AF group (P = 0.051). Cleaved caspase-1 also showed significant correlation with the expression of interleukin-1β and its cleaved form in the total population and in the AF group (P < 0.05). The presence of myocardial and epicardial macrophages were assessed by ionized calcium-binding adaptor molecule 1 (Iba1) immunostaining. Number of macrophages showed a tendency towards elevation in the left atrial myocardium and epicardium of AF compared with SR group. The amount of total and interstitial fibrosis was determined on Masson’s trichrome-stained sections. Histological assessment revealed no difference between AF and SR groups in the amount of either total or interstitial fibrosis. Conclusions This is the first study on inflammation-related differences between HF with SR or AF showing elevated inflam- masome activity and enhanced macrophage infiltration in left atrial samples of patients with AF.

Item Type: Article
Uncontrolled Keywords: Heart failure; Atrial fibrillation; Inflammasome; Macrophages; Fibrosis
Subjects: R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában > R850-854 Experimental medicine / kisérleti orvostudomány
Depositing User: Dr. Zoltán Varga
Date Deposited: 25 Sep 2022 05:11
Last Modified: 03 Apr 2023 08:02
URI: http://real.mtak.hu/id/eprint/149647

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