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Multiple mechanisms contribute to fluorometry signals from the voltage-gated proton channel

Papp, Ferenc and Toombes, Gilman E. S. and Pethő, Zoltán and Bagosi, Adrienn and Feher, Adam and Almássy, János and Borrego, Jesús and Kuki, Ákos and Kéki, Sándor and Panyi, György and Varga, Zoltán (2022) Multiple mechanisms contribute to fluorometry signals from the voltage-gated proton channel. COMMUNICATIONS BIOLOGY. ISSN 2399-3642 (Submitted)

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Abstract

Abstract Voltage-clamp fluorometry (VCF) supplies information about the conformational changes of voltage-gated proteins. Changes in the fluorescence intensity of the dye attached to a part of the protein that undergoes a conformational rearrangement upon the alteration of the membrane potential by electrodes constitute the signal. The VCF signal is generated by quenching and dequenching of the fluorescence as the dye traverses various local environments. Here we studied the VCF signal generation, using the Hv1 voltage-gated proton channel as a tool, which shares a similar voltage-sensor structure with voltage-gated ion channels but lacks an ion-conducting pore. Using mutagenesis and lipids added to the extracellular solution we found that the signal is generated by the combined effects of lipids during movement of the dye relative to the plane of the membrane and by quenching amino acids. Our 3-state model recapitulates the VCF signals of the various mutants and is compatible with the accepted model of two major voltage-sensor movements.

Item Type: Article
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Depositing User: dr. Ferenc Papp
Date Deposited: 27 Sep 2022 09:59
Last Modified: 03 Apr 2023 08:03
URI: http://real.mtak.hu/id/eprint/149989

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