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The atopic skin-like microenvironment modulates the T-cell-polarising cytokine production of myeloid dendritic cells, as determined by laser scanning cytometry.

Nagy, Georgina and Doan-Xuan, Quang-Minh and Gáspár, Krisztián and Mócsai, Gábor and Kapitány, Anikó and Törőcsik, Dániel and Bacsó, Zsolt and Gyimesi, Edit and Remenyik, Eva and Bíró, Tamás and Szegedi, Andrea (2014) The atopic skin-like microenvironment modulates the T-cell-polarising cytokine production of myeloid dendritic cells, as determined by laser scanning cytometry. Experimental dermatology, 23 (4). pp. 276-8. ISSN 1600-0625

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Abstract

Because it is not known exactly when or where myeloid dendritic cells (mDCs) acquire their atopic dermatitis (AD)-specific T-cell-polarising ability in patients with this condition, we used laser scanning cytometry (LSC) to determine whether isolated peripheral blood mDCs from AD patients differed from cells from controls in their cytokine expression profiles de novo and after stimulation with Staphylococcus enterotoxin B (SEB) and thymic stromal lymphopoietin (TSLP), which represents an AD-like microenvironment. Unstimulated mDCs from AD patients showed pluripotent T-cell-polarising capacity, and the surrounding skin microenvironment was essential for the distinctive, disease-specific activity of mDCs (Th2-Th22 bias). We also emphasise that LSC is an attractive technique to study the effect of new DC-targeted therapeutic modalities in AD.

Item Type: Article
Subjects: R Medicine / orvostudomány > RL Dermatology / bőrgyógyászat
Depositing User: Dr Daniel Torocsik
Date Deposited: 17 Sep 2014 13:09
Last Modified: 17 Sep 2014 13:09
URI: http://real.mtak.hu/id/eprint/15125

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