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Integrons and antimicrobial resistance genes of multidrug resistant Escherichia coli and coliform bacteria from foods of animal origin confiscated at the Hungarian borders

Kugler, Renáta and Szmolka, Ama and Pászti, Judit and Nagy, Béla (2014) Integrons and antimicrobial resistance genes of multidrug resistant Escherichia coli and coliform bacteria from foods of animal origin confiscated at the Hungarian borders. In: European Symposium on Food Safety, 2014. május 7-9, Budapest.

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Abstract

The import of contaminated food may represent a food safety risk by the spread of pathogenic and/or multidrug resistant (MDR) bacteria and their determinants for virulence and antimicrobial resistance. Here we aimed to isolate and characterize MDR E. coli and coliform bacteria from food samples from non-Schengen countries confiscated at the Hungarian borders. E. coli and coliform colonies were isolated based on their phenotype on Chromocult® Coliform selective media. Furthermore, API®, PCR and 16S rDNS sequencing were used for species identification. Resistance phenotypes were determined by disc diffusion method for 18 antimicrobials with animal and human clinical relevance. Corresponding antimicrobial resistance and virulence gene patterns were identified using PCR microarray systems AMR05 and Ec03 respectively. The gene cassette arrangements of the integrons were defined by amplicon sequencing. From the total of 207 confiscated food samples 833 coliform isolates were collected. Among them 17 (13 E. coli and 4 coliforms identified as Enterobacter spp.) showed resistance to at least three different antimicrobial classes thus were designated as MDR. The 17 strains represented 14 different food samples. Resistance genes strA, strB, sul2, blaTEM-1, tet(A) predominantly occurred, but in general the prevalence of the virulence genes was low. The identification of genes qnrB, aac(6’)-Ib, blaOXA-7 in some of the isolates indicated the presence of certain emerging antimicrobial resistance plasmids. Class 1 integrons were found in 10 of the 17 MDR isolates (9 E. coli, 1 coliform), and in the majority of them the sul1 gene was absent from their 3’ conserved segment (CS). Interestingly, in one of the pork samples we detected a non-typical class 1 integron carrying the sul3gene on its 3’CS. Above results showed that these illegal foods may frequently carry MDR E. coli and coliform bacteria with some unusual or new antimicrobial resistance traits.

Item Type: Conference or Workshop Item (Poster)
Additional Information: A poszter a konferencia "Ifjúsági Legjobb Poszter" díját nyerte el.
Subjects: S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: PhD Ama Szmolka
Date Deposited: 19 Sep 2014 10:21
Last Modified: 03 Apr 2023 08:13
URI: http://real.mtak.hu/id/eprint/15490

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