REAL

RNA-seq analysis of the peduncle development of Rht12 dwarf plants and primary mapping of Rht12 in common wheat

Chen, L. and Yang, Y. and Mishina, K. and Cui, C. and Zhao, Z. and Duan, S. and Chai, Y. and Su, R. and Chen, F. and Hu, Y. G. (2020) RNA-seq analysis of the peduncle development of Rht12 dwarf plants and primary mapping of Rht12 in common wheat. CEREAL RESEARCH COMMUNICATIONS, 48 (2). pp. 139-147. ISSN 0133-3720 (print), 1788-9170 (online)

[img] Text
CRC_48_139-147.pdf
Restricted to Repository staff only

Download (2MB)

Abstract

Rht12, a dominant dwarfng gene that reduces plant height by approximately 40.0% and substantially decreases peduncle development by 45.0%, has been suggested as an alternative dwarfng gene for wheat improvement. However, the mechanism of dwarf caused by Rht12 is unclear and efcient molecular markers of Rht12 is also lacking in breeding. To explore candidate genes involved in peduncle elongation and to construct a genetic map of Rht12, RNA-seq analysis was applied using the peduncle samples at heading stages of the tall and dwarf residual heterozygous lines derived from the cross of Ningchun45 (rht12rht12) and Karcagi12 (Rht12Rht12). Diferentially expressed genes were screened and annotated, genes involved in polyamine oxidation, carotenoid biosynthesis, protein ubiquitination process and ascorbate pathway were found to be associated with peduncle elongation. Moreover, ten KASP markers on chromosome 5AL closely linked with Rht12 were developed using the SNPs from the RNA-seq data with an F2 mapping population. Furthermore, these markers were mapped to the wheat genome sequence and Rht12 was predicted within the region of 673.7–689.9 Mb on chromosome 5AL. These results provide a better understanding of the molecular mechanisms underlying the development of the peduncle and other stems in wheat, especially in Rht12 dwarf plants, and also promote fne mapping of Rht12.

Item Type: Article
Additional Information: MTA KFB támogatási szerződés alapján archiválva
Uncontrolled Keywords: Wheat, Plant height, Rht12, RNA-seq, KASP marker, Genetic map
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Q Science / természettudomány > QH Natural history / természetrajz > QH426 Genetics / genetika, örökléstan
S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában
Depositing User: Katalin Andódy
Date Deposited: 21 Feb 2023 08:48
Last Modified: 21 Feb 2023 08:48
URI: http://real.mtak.hu/id/eprint/159552

Actions (login required)

Edit Item Edit Item