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Weighted gene co-expression analysis for identification of key genes regulating heat stress in wheat

Mishra, Dwijesh Chandra and Arora, Devender and Kumar, Ranjeet Ranjan and Goswami, Suneha and Varshney, Shivangi and Budhlakoti, Neeraj and Kumar, Sanjeev and Chaturvedi, Krishna Kumar and Sharma, Anu and Chinnusamy, Viswanathan and Rai, Anil (2021) Weighted gene co-expression analysis for identification of key genes regulating heat stress in wheat. CEREAL RESEARCH COMMUNICATIONS, 49 (1). pp. 73-81. ISSN 0133-3720 (print), 1788-9170 (online)

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Abstract

Wheat is an important cereal crop, which holds the second rank globally in terms of production after maize. However, its productivity is highly sensitive to heat stress, which is one of the most serious threats due to global warming. Therefore, development of heat tolerance variety of wheat through molecular breeding approach is an urgent need of the hour for not only reducing productivity loss but also improving crop yield for feeding growing population. In this context, identification of heat-related genes is the first step for this molecular breeding. In this regard, several studies have been conducted in the past, but due to identification of large number of genes, it was found to be practically difficult to use these in molecular breeding programs. In order to address this issue, in this study, system biology approach has been followed to identify set of key genes related to heat stress in wheat which contributes significantly to regulating this entire process. Here, high-throughput RNAseq data were generated using control and treated samples of two contrasting wheat varieties, namely HD2967 (thermo-tolerant) and BT-Schomburgk (thermo-susceptible). Further, in order to identify important key genes, an advanced statistical framework called weighted gene co-expression network analysis (WGCNA) has been used. Moreover, functional annotation of these identified key genes has also been carried out, which confirms their association with the heat stress. These results will provide important lead to experimenters involved in the development of new heat-stress-tolerant wheat cultivars to mitigate effects of global warming.

Item Type: Article
Additional Information: MTA KFB támogatási szerződés alapján archiválva
Uncontrolled Keywords: Co-expression analysis, Global warming, Heat stress, Transcriptome, Gene regulatory network, Key genes
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Q Science / természettudomány > QH Natural history / természetrajz > QH426 Genetics / genetika, örökléstan
S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában
Depositing User: Katalin Andódy
Date Deposited: 07 Mar 2023 11:51
Last Modified: 07 Mar 2023 11:51
URI: http://real.mtak.hu/id/eprint/161609

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