REAL

Reconsidering Dogmas about the Growth of Bacterial Populations

Ughy, Bettina and Nagyapáti, Sarolta and Lajko, Dezi B. and Letoha, Tamas and Prohaszka, Adam and Deeb, Dima and Dér, András and Pettkó-Szandtner, Aladár and Szilák, László (2023) Reconsidering Dogmas about the Growth of Bacterial Populations. CELLS, 12 (10). ISSN 2073-4409

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Abstract

The growth of bacterial populations has been described as a dynamic process of continuous reproduction and cell death. However, this is far from the reality. In a well fed, growing bacterial population, the stationary phase inevitably occurs, and it is not due to accumulated toxins or cell death. A population spends the most time in the stationary phase, where the phenotype of the cells alters from the proliferating ones, and only the colony forming unit (CFU) decreases after a while, not the total cell concentration. A bacterial population can be considered as a virtual tissue as a result of a specific differentiation process, in which the exponential-phase cells develop to stationary-phase cells and eventually reach the unculturable form. The richness of the nutrient had no effect on growth rate or on stationary cell density. The generation time seems not to be a constant value, but it depended on the concentration of the starter cultures. Inoculations with serial dilutions of stationary populations reveal a so-called minimal stationary cell concentration (MSCC) point, up to which the cell concentrations remain constant upon dilutions; that seems to be universal among unicellular organisms.

Item Type: Article
Uncontrolled Keywords: population growth; stationary phase; exponential phase; differentiation; transition; generation time; proteomics
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3020 Biophysics / biofizika
Q Science / természettudomány > QK Botany / növénytan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 23 May 2023 06:16
Last Modified: 23 May 2023 06:16
URI: http://real.mtak.hu/id/eprint/165767

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