Hephaestia caeni gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from activated sludge

Felföldi, Tamás and Vengring, Anita and Kéki, Zsuzsa and Márialigeti, Károly and Schumann, Peter and M. Tóth, Erika (2014) Hephaestia caeni gen. nov., sp. nov., a novel member of the family Sphingomonadaceae isolated from activated sludge. International Journal of Systematic and Evolutionary Microbiology, 64. pp. 1920-1925. ISSN 1466-5026

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Abstract

A Gram-staining-negative, rod-shaped and motile bacterium, designated strain ERB1-3T, was isolated from a laboratory-scale activated sludge system treating coke plant effluent using thiocyanate-supplemented growth medium. Strain ERB1-3T was oxidase-positive and weakly catalase-positive. The predominant fatty acids were C18 : 1v7c (35.6 %) and C17 : 1v6c (29.2 %), and the major respiratory quinone was Q-10. Polar lipids were dominated by sphingoglycolipid and phosphatidylglycerol. Major polyamines were spermidine and sym-homospermidine. The G+C content of the genomic DNA of strain ERB1-3T was 66.4 mol%. Based on the 16S rRNA gene, strain ERB1-3T exhibited the highest sequence similarity values to Sphingomonas sanxanigenens DSM 19645T (96.1 %), Sphingobium scionense DSM 19371T (95.1 %) and Stakelama pacifica LMG 24686T (94.8 %) within the family Sphingomonadaceae. The novel isolate had some unique chemotaxonomic features that differentiated it from these closely related strains, contained much more C17 : 1v6c, C15 : 0 2-OH, C17 : 0 and C17 : 1v8c fatty acids and possessed diphosphatidylglycerol only in trace amounts. On the basis of the phenotypic, chemotaxonomic and molecular data, strain ERB1-3T is considered to represent a novel genus and species, for which the name Hephaestia caeni gen. nov., sp. nov. is proposed. The type strain is ERB1-3T (5DSM 25527T5NCAIM B 02511T).

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