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Transient receptor potential vanilloid-2 mediates the effects of transient heat shock on endocytosis of human monocyte-derived dendritic cells

Szöllősi, Attila and Oláh, Attila and Tóth, Balázs István and Papp, Ferenc and Czifra, Gabriella and Panyi, György and Bíró, Tamás (2013) Transient receptor potential vanilloid-2 mediates the effects of transient heat shock on endocytosis of human monocyte-derived dendritic cells. FEBS LETTERS, 587 (9). pp. 1440-1445. ISSN 0014-5793

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Abstract

Our goal was to investigate the effect of heat shock on human monocyte-derived dendritic cells (DCs) and to dissect the role of thermosensitive transient receptor potential (TRP) channels in the process. We provide evidence that a short heat shock challenge (43 degrees C) decreased the endocytotic activity of the DCs and that this effect could be alleviated by the RNAi-mediated knockdown of TRPV2 but, importantly, not by the pharmacological (antagonists) or molecular (RNAi) suppression of TRPV1 and TRPV4 activities/levels. Likewise, the heat shock-induced robust membrane currents were selectively and markedly inhibited by TRPV2 "silencing" whereas modulation of TRPV1 and TRPV4 activities, again, had no effect. These intriguing data introduce TRPV2-coupled signaling as a key player in mediating the cellular actions of heat shock on DCs.

Item Type: Article
Uncontrolled Keywords: Time Factors; TRPV Cation Channels/genetics/*metabolism; Monocytes/*cytology; Humans; *Heat-Shock Response; gene expression regulation; *Endocytosis; Electrophysiological Processes; Dendritic Cells/*cytology/*metabolism; Cell Membrane/metabolism
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 13 Feb 2015 14:59
Last Modified: 13 Feb 2015 14:59
URI: http://real.mtak.hu/id/eprint/21741

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