Effect-Directed Isolation and Identification of Antibacterial Chelidonium majus L. Alkaloids

Móricz, Ágnes and Fornal, Emilia and Jesionek, Wioleta and Majer-Dziedzic, Barbara and Choma, Irena M. (2015) Effect-Directed Isolation and Identification of Antibacterial Chelidonium majus L. Alkaloids. CHROMATOGRAPHIA, 78 (9-10). pp. 707-716. ISSN 0009-5893

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Abstract

The components of alkaloid, methanol and ethyl acetate extracts of Chelidonium majus L. root, leaf and stem were tested for antibacterial activity against Bacillus subti - lis and Escherichia coli by thin-layer chromatography cou - pled with direct bioautography (TLC-DB). Effect-directed analyses were performed to obtain the active compounds as follows: separation with preparative layer chromatogra - phy (PLC, 0.5-mm-thick silica layer, 20 × 10 cm); a part of the preparative layer (3 cm wide) was used for PLC-DB to detect the antibacterial components against B. subtilis ; elu - tion of the appropriate lanes as indicated by PLC-DB from the remaining 17-cm-wide layer; verification of the bioac - tivity of the eluted compounds by TLC-DB; further purifi - cation of the fractions was carried out with TLC, when it was necessary. The eluates of interest were analysed by LC-HRMS and LC-Q/TOF. The antibacterial effect against B. subtilis was mainly attributed to the isoquinoline alka - loid components, which were identified as chelidonine, sanguinarine, chelerythrine, berberine, coptisine, corydine, dihydrosanguinarine, dihydrochelerythrine, 6-acetonyl- dihydrosanguinarine, 6-acetonyl-dihydrochelerythrine and stylopine. Only chelidonine, sanguinarine and chelerythrine possessed inhibiting effect against E. coli .

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