Nuclear position dictates DNA repair pathway choice.

Lemaître, Charlène and Grabarz, Anastazja and Tsouroula, Katerina and Andronov, Leonid and Furst, Audrey and Pankotai, Tibor and Heyer, Vincent and Rogier, Mélanie and Attwood, Kathleen M. and Kessler, Pascal and Dellaire, Graham and Klaholz, Bruno and Reina-San-Martin, Bernardo and Soutoglou, Evi (2014) Nuclear position dictates DNA repair pathway choice. Genes & development, 28 (22). pp. 2450-63. ISSN 1549-5477

Genes Dev.-2014-Lemaître-2450-63.pdf

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Faithful DNA repair is essential to avoid chromosomal rearrangements and promote genome integrity. Nuclear organization has emerged as a key parameter in the formation of chromosomal translocations, yet little is known as to whether DNA repair can efficiently occur throughout the nucleus and whether it is affected by the location of the lesion. Here, we induce DNA double-strand breaks (DSBs) at different nuclear compartments and follow their fate. We demonstrate that DSBs induced at the nuclear membrane (but not at nuclear pores or nuclear interior) fail to rapidly activate the DNA damage response (DDR) and repair by homologous recombination (HR). Real-time and superresolution imaging reveal that DNA DSBs within lamina-associated domains do not migrate to more permissive environments for HR, like the nuclear pores or the nuclear interior, but instead are repaired in situ by alternative end-joining. Our results are consistent with a model in which nuclear position dictates the choice of DNA repair pathway, thus revealing a new level of regulation in DSB repair controlled by spatial organization of DNA within the nucleus.

Item Type: Article
Subjects: Q Science / természettudomány > Q1 Science (General) / természettudomány általában
Depositing User: Dr Pankotai Tibor
Date Deposited: 09 Sep 2015 07:43
Last Modified: 09 Sep 2015 07:43

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