The high-performance liquid chromatography/multistageelectrospray mass spectrometric investigation and extractionoptimization of beech (Fagus sylvatica L.) bark polyphenols

Hofmann, Tamás and Nebehaj, Esztella and Albert, Levente (2015) The high-performance liquid chromatography/multistageelectrospray mass spectrometric investigation and extractionoptimization of beech (Fagus sylvatica L.) bark polyphenols. JOURNAL OF CHROMATOGRAPHY A, (1393). pp. 96-105. ISSN 0021-9673

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Abstract

The aim of the present work was the high-performance liquid chromatographic separation and multi-stage mass spectrometric characterization of the polyphenolic compounds of beech bark, as well as theextraction optimization of the identified compounds. Beech is a common and widely used material inthe wood industry, yet its bark is regarded as a by-product. Using appropriate extraction methods thesecompounds could be extracted and utilized in the future.Different extraction methods (stirring, sonication, microwave assisted extraction) using different sol-vents (water, methanol:water 80:20 v/v, ethanol:water 80:20 v/v) and time/temperature schedules havebeen compared basing on total phenol contents (Folin–Ciocâlteu) and MRM peak areas of the identifiedcompounds to investigate optimum extraction efficiency.Altogether 37 compounds, including (+)-catechin, (−)-epicatechin, quercetin-O-hexoside, taxifolin-O-hexosides (3), taxifolin-O-pentosides (4), B-type (6) and C-type (6) procyanidins, syringic acid-and coumaric acid-di-O-glycosides, coniferyl alcohol- and sinapyl alcohol-glycosides, as well as otherunknown compounds with defined [M−H]−m/z values and MS/MS spectra have been tentatively iden-tified.The choice of the method, solvent system and time/temperature parameters favors the extraction ofdifferent types of compounds. Pure water can extract compounds as efficiently as mixtures containingorganic solvents under high-pressure and high temperature conditions. This supports the implementa-tion of green extraction methods in the future. Extraction times that are too long and high temperaturescan result in the decrease of the concentrations.Future investigations will focus on the evaluation of the antioxidant capacity and utilization possibili-ties of the prepared extracts

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