Dual Excitatory and Smooth Muscle-relaxing Effect of Sideritis montana L. Extract on Guinea-pig Ileum

Tóth, Barbara and Barthó, Loránd and Vasas, Andrea and Sándor, Zsolt and Jedlinszki, Nikoletta and Pinke, Gyula and Hohmann, Judit (2014) Dual Excitatory and Smooth Muscle-relaxing Effect of Sideritis montana L. Extract on Guinea-pig Ileum. NATURAL PRODUCT COMMUNICATIONS, 10 (3). pp. 487-490. ISSN 1934-578X

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Abstract

The neuronal and smooth muscle effects of methanol extract prepared from the air-dried flowering aerial parts of Sideritis montana L. (SEM) was tested in vitro on guinea-pig ileum. The chemical composition of the investigated extract was analysed by HPLC-MS, and chrysoeriol, chlorogenic acid and caffeic acid were detected as main constituents. The isolated organ assay showed that S. montana extract caused an immediate contraction and a more slowly developing inhibitory response in the ileum. The SME-induced contractions were strongly inhibited by the acetylcholine muscarinic receptor antagonist atropine (0.5 µM), but not by the Na+ channel blocker tetrodotoxin (TTX; 0.5 µM) or the histamine H1 receptor antagonist chloropyramine (0.5 µM). Selective desensitization of capsaicin-sensitive neurons by the sensory neuron stimulant and blocker capsaicin did not influence the contractile effect of SME. As to the spasmolytic effect, SME inhibited the effects of electrical field stimulation, exogenous acetylcholine, or histamine. These smooth muscle-relaxing effects were reversible by repeated renewals of the bathing solution in 40 min.

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