Tax, Gábor and Urbán, Edit and Puskás, Róbert and Kónya, Zoltán and Kemény, Lajos and Szabó, Kornélia (2014) PROPIONIC ACID SECRETED BY PROPIONIBACTERIUM ACNES MAY MODIFY THE CELLULAR PROPERTIES OF KERATINOCYTES. Immunológiai Szemle, 6 (3-4). p. 48. ISSN 2061-0203/2015.1.


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Propionibacterium acnes (P. acnes) bacterium is a member of the skin microflora, but may also serve as an opportunistic pathogen contributing to the pathogenesis of acne vulgaris. Earlier we have shown that various P. acnes strains (889, 6609, ATCC 11828) belonging to different phylogroups differentially affect the cellular properties of cultured human keratinocytes in a strain-specific and dose-dependent manner. High doses of the pathogenic 889 and ATCC 11828 strains also resulted characteristic morphological changes and membrane damage, which lead to the cytotoxicity of human in vitro cultures keratinocytes (HPV-KER). Our aim was to further analyze the interaction of human in vitro cultured keratinocyes and identify bacterially-derived factors that may mediate the previously observed effects. In order to systematically quantify the P. acnes-induced cytotoxicity we performed spectrophotometric lactate dehydrogenase (LDH) and hemoglobin (HgB) assays using supernatant samples of bacterial treated HPV-KER cells and erythrocytes. The amount of released free LDH and HgB exhibited strain- and dose-dependent differences. We also noted the differential acidification of the pH in the culture supernatants. P. acnes is known to secrete propionic acid (PA), a characteristic, acidic end-product of bacterial fermentation in these species. In order to analyze whether P. acnes-derived PA has any role in the observed cellular changes we treated HPV KER cells with the acid and analyzed the cell morphology. Microscopic analysis of the PA treated cultures revealed cells with similar irregular membrane morphologies observed earlier upon high dose P. acnes 889 and ATCC 11828 treatments. Finally, we measured the amount of secreted short chain fatty acids (SCFA) in the P. acnes 889, 6609 and ATCC 11828-treated HPV-KER supernatant samples by mass spectrometry. These studies revealed marked differences in the amount of secreted PA; high dose treatment of the 889 and ATCC 11828 strains leading to higher levels. P. acnes-induced cellular changes depend on the type and amount of the applied bacterial strains. The observed differences may be due to variations of the amount of a secreted metabolic end-product, PA. Together with other bacterially-derived molecules it may be an active contributor of the P. acnes-induced cellular changes.

Item Type: Article
Additional Information: A MIT 43. Vándorgyűlése. Velence, Magyarország: 2014.10.15 -2014.10.17.
Subjects: R Medicine / orvostudomány > RL Dermatology / bőrgyógyászat
Depositing User: Dr. Kornélia Szabó
Date Deposited: 25 Sep 2015 13:41
Last Modified: 25 Sep 2015 13:41

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