REAL

Crystallization and preliminary crystallographic analysis of dUTPase from the helper phage Φ11 of Staphylococcus aureus

Leveles, Ibolya and Róna, Gergely and Zagyva, Imre and Bendes, Ábris and Harmat, Veronika and Vértessy G., Beáta (2011) Crystallization and preliminary crystallographic analysis of dUTPase from the helper phage Φ11 of Staphylococcus aureus. Acta Crystallogr Sect F Struct Biol Cryst Commun, 67 (11). pp. 1411-1413.

[img]
Preview
PDF
Leveles.pdf

Download (184Kb)

Abstract

Staphylococcus aureus superantigen-carrying pathogenicity islands (SaPIs) have a determinant role in spreading virulence genes among bacterial populations that constitute a major health hazard. Repressor (Stl) proteins are responsible for transcriptional regulation of pathogenicity island genes. Recently, a derepressing interaction between the repressor Stl SaPIbov1 with dUTPase from the Φ11 helper phage was suggested [Tormo-Mas et al. (2010). Nature 465, 779-782]. Towards elucidating the molecular mechanism of this interaction, this study reports expression, purification, and X-ray analysis of Φ11 dUTPase that contains a phage-specific polypeptide segment not present in other dUTPases. Crystals were obtained using the hanging-drop vapor-diffusion method at room temperature. Data were collected from one type of crystal to 2.98 Å resolution. The crystal of Φ11 dUTPase belonged to the cubic space group I23, with unit-cell parameters a=98.16 Å, α=β=γ= 90.00o.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Depositing User: Prof Gábor Náray-Szabó
Date Deposited: 23 Feb 2012 10:58
Last Modified: 17 Oct 2013 12:26
URI: http://real.mtak.hu/id/eprint/2904

Actions (login required)

View Item View Item