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Isolation and characterisation of flavobacteria from wild and cultured freshwater fishes in Hungary

Varga, Zsuzsanna and Sellyei, Boglárka and Paulus, Petra and Papp, Melitta and Molnár, Kálmán and Székely, Csaba (2015) Isolation and characterisation of flavobacteria from wild and cultured freshwater fishes in Hungary. Acta Veterinaria Hungarica, 64 (1). pp. 13-25. ISSN 0236-6290 (print), 1588-2705 (online)

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Abstract

This study surveyed the prevalence of Flavobacterium columnare in wild and cultured freshwater fishes in Hungary. This bacterium usually causes disease in waters of more than 25°C temperature. However, with the introduction of intensive fish farming systems, infected fish exposed to stress develop disease signs also at lower temperatures; in addition, the temperature of natural waters rises to the critical level due to global warming. Twenty-five isolates from ulcerous fish stocks were identified as F. columnare by specific PCR, although both the fragment lengths and the results of PCR-RFLP genotyping with BsuRI (HaeIII) and RsaI restriction enzymes raised doubts regarding this species classification. Sequencing of the 16S ribosomal RNA gene revealed that 23 isolates belonged to the species F. johnsoniae and 2 represented Chryseobacterium spp. The isolates were found to have high-level multidrug resistance: all were resistant to ampicillin and polymyxin B, the 23 F. johnsoniae strains to cotrimoxazole, 88% of them to gentamicin and 72% to chloramphenicol. The majority of the 25 isolates were sensitive to erythromycin (88%), enrofloxacin (84%), furazolidone (76%) and florfenicol (68%).

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: Boglárka Sellyei
Date Deposited: 03 Nov 2015 09:07
Last Modified: 13 Jan 2017 15:33
URI: http://real.mtak.hu/id/eprint/30040

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