Szigeti, Gyula P. (2012) NICOTINE INTERFERES WITH PURINERGIC SIGNALING IN SMOOTH MUSCLE CELLS ISOLATED FROM URINARY BLADDERS OF PATIENTS WITH OUTLET OBSTRUCTION. Neurourology and Urodinamics. (Submitted)
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Aim. In patients with outlet obstruction, the contraction of the base is reduced compared to that of healthy individuals, while the contraction of the dome is not affected. Here, we investigated the cellular mechanisms that might be responsible for cholinergic effects blocking non-adrenergic non-cholinergic contractions in the base of the urinary bladder. Methods. Smooth muscle cells from the base and from the dome of human urinary bladders with or without outlet obstruction were cultured to determine the contribution of cholinergic and purinergic mechanisms to their Ca2+ homeostasis. Results. While ATP evoked Ca2+ transients in all the cells, nicotine and carbachol induced Ca2+ transients only in 56% and 44% of the cells, respectively. When ATP was administered together with nicotine or carbachol, the amplitudes of the Ca2+ transients recorded from cells prepared from the base of bladders with outlet obstruction were significantly smaller (29±7% with nicotine and 54±10% with carbachol) than those evoked by ATP alone. This cross-inhibition was much less apparent in the dome of bladders with outlet obstruction and in the base of healthy bladders. The presence of nAChRα3 and nAChRα7 subunits and P2X2 receptors was demonstrated using immunocytochemistry and immunohistochemistry in smooth muscle cell cultures and tissue sections from urinary bladders. Conclusion. The cross-inhibition between the cholinergic and purinergic signaling pathways reported in this work may decrease the strength of the contraction of the base of the urinary bladder in patients with outlet obstruction during voiding.
|Subjects:||R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában|
|Depositing User:||Dr. Gyula P Szigeti|
|Date Deposited:||26 Sep 2012 11:24|
|Last Modified:||15 May 2013 04:21|
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