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Cytotoxic T lymphocyte (CTL) responses to human cytomegalovirus pp65, IE1-exon4, gB, pp150, and pp28 in healthy individuals: Reevaluation of prevalence of IE1-specific CTLs

Gyulai, Zsófia and Endrész, Valéria and Burián, Katalin and Pincus, Steve and Toldy, József and Cox, William I. and Meric, Claude and Plotkin, Stanley and Gönczöl, Éva and Berencsi, Klára (2000) Cytotoxic T lymphocyte (CTL) responses to human cytomegalovirus pp65, IE1-exon4, gB, pp150, and pp28 in healthy individuals: Reevaluation of prevalence of IE1-specific CTLs. Journal of Infectious Diseases, 181. pp. 1537-1546. ISSN 0022-1899 (print), 1537-6613 (online)

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Abstract

The prevalence of human cytomegalovirus (HCMV) pp65-, pp150-, IE1-exon4–, gB- and pp28-specific cytotoxic T lymphocyte (CTL) responses was compared among 34 healthy individuals, grouped by neutralizing antibody titers.Moderately and highly seropositive donors showed predominantly pp65- and IE1-exon4–specific CTL responses (92% and 76% of the donors, respectively), with similar precursor frequencies in the 2 donors tested. In addition, highly seropositive and a few moderately seropositive donors showed CTL responses to gB and pp150 (33% and 30% of the donors, respectively). No individual recognized pp28 as a target in the CTL assay. Phenotypic analysis revealed a mixed effector population of CD4+ and CD8+ (1 donor) or only CD8+ cells for pp65-specific effectors (2 donors). IE1-exon4– and pp150-specific effectors were CD8+ (2 donors and 1 donor, respectively), whereas gB- specific CTLs were CD4+ (1 donor). These data may help to design a cellular immunity-based vaccine effective against HCMV diseases.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
R Medicine / orvostudomány > RZ Other systems of medicine / orvostudomány egyéb területei
Depositing User: Erika Bilicsi
Date Deposited: 10 Oct 2012 09:53
Last Modified: 10 Oct 2012 09:54
URI: http://real.mtak.hu/id/eprint/3065

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