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GUcal: An integrated application for capillary electrophoresis based glycan analysis

Járvás, Gábor and Szigeti, Márton and Guttman, András (2015) GUcal: An integrated application for capillary electrophoresis based glycan analysis. ELECTROPHORESIS, 36 (24). pp. 3094-3096. ISSN 0173-0835, ESSN: 1522-2683

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Abstract

Recent emergence in the use of monoclonal antibody therapeutics and other glycoprotein biopharmaceuticals requires high-throughput, robust, and automated techniques for their glycosylation analysis. Capillary electrophoresis is one of the high-performancemethods of choice; however, while the necessary instrumentation is well developed, the related bioinformatics tools are lacked behind. In this paper, we introduce an integrated toolset dubbed as GUcal, to automatically calculate the glucose unit (GU) values for all sample components of interest in an electropherogram with a concomitant database search for structural assignment. The database comprises CEGUs and suggested structures ofN-glycans released from human IgG. The app is freely available online (www.lendulet.uni-pannon.hu/gucal)and readily facilitates CE-based glycan analysis.

Item Type: Article
Subjects: Q Science / természettudomány > QD Chemistry / kémia
Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
Depositing User: László Hajba
Date Deposited: 12 Jan 2016 15:16
Last Modified: 12 Jan 2016 15:16
URI: http://real.mtak.hu/id/eprint/32101

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