Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition

Jetzschmann, Katharina J. and Jágerszki, Gyula and Dechtrirat, Decha and Yarman, A. and Gajovic-Eichelmann, Nenad and Gyurcsányi, Ervin Róbert (2015) Vectorially Imprinted Hybrid Nanofilm for Acetylcholinesterase Recognition. ADVANCED FUNCTIONAL MATERIALS, 25 (32). pp. 5178-5183. ISSN 1616-301X


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Effective recognition of enzymatically active tetrameric acetylcholinesterase (AChE) is accomplished by a hybrid nanofilm composed of a propidium-terminated self-assembled monolayer (Prop-SAM) which binds AChE via its peripheral anionic site (PAS) and an ultrathin electrosynthesized molecularly imprinted polymer (MIP) cover layer of a novel carboxylate-modified derivative of 3,4-propylenedioxythiophene. The rebinding of the AChE to the MIP/Prop-SAM nanofilm covered electrode is detected by measuring in situ the enzymatic activity. The oxidative current of the released thiocholine is dependent on the AChE concentration from ≈0.04 × 10−6 to 0.4 × 10−6m. An imprinting factor of 9.9 is obtained for the hybrid MIP, which is among the best values reported for protein imprinting. The dissociation constant characterizing the strength of the MIP-AChE binding is 4.2 × 10−7m indicating the dominant role of the PAS-Prop-SAM interaction, while the benefit of the MIP nanofilm covering the Prop-SAM layer is the effective suppression of the cross-reactivity toward competing proteins as compared with the Prop-SAM. The threefold selectivity gain provided by i) the “shape-specific” MIP filter, ii) the propidium-SAM, iii) signal generation only by the AChE bound to the nanofilm shows promise for assessing AChE activity levels in cerebrospinal fluid.

Item Type: Article
Uncontrolled Keywords: Propidium; peripheral anionic site; molecularly imprinted electropolymers; Biomimetic sensors; ACETYLCHOLINESTERASE
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Depositing User: MTMT SWORD
Date Deposited: 04 Feb 2016 13:44
Last Modified: 14 Jul 2016 23:15

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