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ISOLATION AND CHARACTERIZATION OF AN R-PRIME PLASMID FROM RHIZOBIUM-MELILOTI

Kiss, György Botond and Dobo, Katalin and Dusha, Ilona and Breznovits, Ágnes and Orosz, László and Vincze, Éva and Kondorosi, Ádám (1980) ISOLATION AND CHARACTERIZATION OF AN R-PRIME PLASMID FROM RHIZOBIUM-MELILOTI. Journal of Bacteriology, 141 (1). pp. 121-128. ISSN 0021-9193 (print), 1098-5530 (online)

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Abstract

Using a simple enrichment procedure, we isolated an R-prime derivative of plasmid R68.45 carrying a 17.8-megadalton segment of the Rhizobium meliloti 41 chromosome. The chromosomal segment carried on this plasmid (pGY1) includes the markers cys-24+, cys-46, and att,s6. Plasmid pGY1 mobilized the chromosome in a polarized way starting from the region of homology, but cannot promote chromosome transfer from other sites. The attlsC3 site on pGY1 allowed the integration of phage 16-3 into pGY1, and a composite plasmid of 91.8 megadaltons was formed. This vector (pGY2) is suitable for the introduction of Rhizobium bacteriophage 16-3 into other gram-negative bacteria.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH426 Genetics / genetika, örökléstan
Q Science / természettudomány > QK Botany / növénytan
Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Erika Bilicsi
Date Deposited: 13 Nov 2012 08:05
Last Modified: 13 Nov 2012 08:05
URI: http://real.mtak.hu/id/eprint/3345

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