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Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium — Short communication

Nagy, Szabolcs and Kakasi, Balázs and Bercsényi, Miklós (2016) Flow cytometric detection of oxidative DNA damage in fish spermatozoa exposed to cadmium — Short communication. Acta Veterinaria Hungarica, 64 (1). pp. 120-124. ISSN 0236-6290

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Abstract

The aim of the present pilot study was to apply a flow cytometric assay, the so-called OxyDNA test, to determine the level of oxidative DNA damage in fish spermatozoa exposed to different concentrations (0.01–10,000 mg/L) of cadmium. Milt was collected from three randomly selected Prussian carp (Carassius auratus gibelio) males. Oxidative DNA damage was assessed with the OxyDNA kit and using flow cytometry. The ratio of OxyDNA-positive events increased significantly at higher cadmium concentrations. The results indicate that direct contact of fish spermatozoa with cadmium-polluted water initiates genotoxic damage.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: Erika Bilicsi
Date Deposited: 02 Jun 2016 04:29
Last Modified: 31 Mar 2017 23:15
URI: http://real.mtak.hu/id/eprint/35571

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