Development of a multiplex event-specific PCR assay for detection of genetically modified rice

Lu, J. and Ji, G.Z. and Li, G. and Wu, Y.F. and Yang, J. and Lin, S.L. and Yang, D.L. and Zhao, J.N. and Xiu, W.M. (2016) Development of a multiplex event-specific PCR assay for detection of genetically modified rice. Cereal Research Communications, 44 (1). pp. 47-56. ISSN 0133-3720

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Abstract

Global rice supplies have been found contaminated with unapproved varieties of genetically modified (GM) rice in recent years, which has led to product recalls in several of countries. Faster and more effective detection of GM contamination can prevent adulterated food, feed and seed from being consumed and grown, minimize the potential environmental, health or economic damage. In this study, a simple, reliable and cost-effective multiplex polymerase chain reaction (PCR) assay for identifying genetic modifications of TT51-1, Kemingdao1 (KMD1) and Kefeng6 (KF6) rice was developed by using the event-specific fragment. The limit of detection (LOD) for each event in the multiplex PCR is approximately 0.1%. Developed multiplex PCR assays can provide a rapid and simultaneous detection of GM rice.

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