Calcium signaling in human pluripotent stem cells

Apáti, Ágota and Berecz, Tünde and Sarkadi, Balázs (2016) Calcium signaling in human pluripotent stem cells. Cell Calcium, 59 (2-3). pp. 117-123. ISSN 0143-4160


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Human pluripotent stem cells provide new tools for developmental and pharmacological studies as well as for regenerative medicine applications. Calcium homeostasis and ligand-dependent calcium signaling are key components of major cellular responses, including cell proliferation, differentiation or apoptosis. Interestingly, these phenomena have not been characterized in detail as yet in pluripotent human cell sates. Here we review the methods applicable for studying both short- and long-term calcium responses, focusing on the expression of fluorescent calcium indicator proteins and imaging methods as applied in pluripotent human stem cells. We discuss the potential regulatory pathways involving calcium responses in hPS cells and compare these to the implicated pathways in mouse PS cells. A recent development in the stem cell field is the recognition of so called "naïve" states, resembling the earliest potential forms of stem cells during development, as well as the "fuzzy" stem cells, which may be alternative forms of pluripotent cell types, therefore we also discuss the potential role of calcium homeostasis in these PS cell types. © 2016 Elsevier Ltd.

Item Type: Article
Additional Information: N1 Funding Details: K115375, OTKA, Hungarian Scientific Research Fund
Uncontrolled Keywords: Pluripotent Stem Cells; Pluripotency maintenance; Naïve and primed state; Genetically engineered calcium indicators; Calcium signals; Calcium sensitive dyes
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3020 Biophysics / biofizika
Depositing User: MTMT SWORD
Date Deposited: 08 Jun 2016 14:04
Last Modified: 08 Jun 2016 14:04

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