Diversity of Cryphonectria parasitica populations from the Carpathian Basin

Görcsös, Gábor and Irinyi, László and Radócz, László and Tarcali, Gábor and Sándor, Erzsébet (2015) Diversity of Cryphonectria parasitica populations from the Carpathian Basin. Acta Microbiologica et Immunologica Hungarica, 62 (3). pp. 247-266. ISSN 1217-8950


Download (431kB) | Preview


The ascomycetous fungus Cryphonectria parasitica (Murr.) is one of the most important fungal pathogens of chestnut (Castanea sativa Mill.), causing chestnut blight. It is originated from Eastern Asia and was introduced into North America, and later into Europe. Almost 100 C. parasitica isolates were collected mainly from the Carpathian Basin to study their diversity and phylogenetic relationships. Three nuclear molecular markers were tested: (i) internal transcribed spacers (ITS), (ii) translation elongation factor 1 subunit alpha (tef1), and (iii) six microsatellite loci. The ITS region proved to be highly polymorphic but this kind of variability did not reflect the geographical regions of the isolates, while the tef1 sequences were identical in all isolates. Microsatellite haplotype diversity was relatively high (0.923–0.984) calculated for the fungal populations of the Carpathian Basin. This may have resulted from the multiple introductions from diverse origins of the populations, close to the first site of observation in Northern Italy. The BAPS analysis of the microsatellite markers occasionally grouped together samples from remote geographical origin, suggesting human assistance in the introduction of new C. parasitica haplotypes from distinct areas.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 30 Jun 2016 12:11
Last Modified: 30 Sep 2016 23:15

Actions (login required)

Edit Item Edit Item