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Effect of antimicrobial peptides on colistin-susceptible and colistin-resistant strains of Klebsiella pneumoniae and Enterobacter asburiae

Kádár, Béla and Kocsis, Béla and Kristof, Katalin and Tóth, Ákos and Szabó, Dóra (2015) Effect of antimicrobial peptides on colistin-susceptible and colistin-resistant strains of Klebsiella pneumoniae and Enterobacter asburiae. Acta Microbiologica et Immunologica Hungarica, 62 (4). pp. 501-508. ISSN 1217-8950

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Abstract

In this study susceptibility to different antimicrobial peptides was investigated on colistin-susceptible and colistin-resistant identical pulsotype strains of KPC-2 producing Klebsiella pneumoniae ST258 as well as colistin-susceptible and colistin-resistant Enterobacter asburiae strains isolated from clinical samples. In our test, bacteria were exposed to 50 mg/ml lactoferrin, lysozyme and protamine — cationic antimicrobial peptides belonging to innate immune system and having structural similarity to polymyxins — in separate reactions. After 18 hours incubation of colonies were counted. 40% of colistin-resistant K. pneumoniae strains and 97% of colistin-susceptible counterpart strains were lysed by protamine whereas 87% and 100% colony forming unit decrease by lysozyme was seen, respectively. In the case of colistin-resistant E. asburiae strains 1 log<sub>10</sub> cell count increase were observed after treatment with lysozyme and 1.56 log<sub>10</sub> after lactoferrin exposure compared to the initial number whereas the colistin-susceptible showed no relevant cell count increase. Our findings suggest that acquired colistin-resistance in Enterobacteriaceae is associated with tolerance against antimicrobial peptides.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 30 Jun 2016 12:40
Last Modified: 31 Dec 2016 00:15
URI: http://real.mtak.hu/id/eprint/36920

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